ArticleJournal of cancer research and clinical oncology1994
Ascorbic-acid-mediated iron release from cellular ferritin and its relation to the formation of DNA strand breaks in neuroblastoma cells.
Article in Journal of cancer research and clinical oncology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 46 citations in OpenAlex.
- The Role of Glia in Wilson's Disease: Clinical, Neuroimaging, Neuropathological and Molecular Perspectives.International journal of molecular sciences · 2024Review
- Role of endolysosome function in iron metabolism and brain carcinogenesis.Seminars in cancer biology · 2021Review
- Efficiency of long-term high-dose intravenous ascorbic acid therapy in locally advanced basal cell carcinoma - a pilot study.Postepy dermatologii i alergologii · 2020Article
- Radiation Induced Metabolic Alterations Associate With Tumor Aggressiveness and Poor Outcome in Glioblastoma.Frontiers in oncology · 2020Article
- A systematic review and meta-analysis on the association of serum and tumor tissue iron and risk of breast cancer.Caspian journal of internal medicine · 2020Review
- High Dose Ascorbate Causes Both Genotoxic and Metabolic Stress in Glioma Cells.Antioxidants (Basel, Switzerland) · 2017Article
- Brain iron overload following intracranial haemorrhage.Stroke and vascular neurology · 2016Review
- Computational modeling and analysis of iron release from macrophages.PLoS computational biology · 2014Article
- Pharmacological doses of daily ascorbate protect tumors from radiation damage after a single dose of radiation in an intracranial mouse glioma model.Frontiers in oncology · 2014Article
- Pharmacological ascorbate and ionizing radiation (IR) increase labile iron in pancreatic cancer.Redox biology · 2013Article
- A survey on herbal management of hepatocellular carcinoma.World journal of hepatology · 2011Article
- Brain iron toxicity: differential responses of astrocytes, neurons, and endothelial cells.Neurochemical research · 2007Review
- Dietary vitamin C supplementation decreases blood pressure in DOCA-salt hypertensive male Sprague-Dawley rats and this is associated with increased liver oxidative stress.Molecular and cellular biochemistry · 2002Article
- Intracellular ascorbic acid enhances the DNA single-strand breakage and toxicity induced by peroxynitrite in U937 cells.The Biochemical journal · 2001Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ascorbic acid at pharmacologically attainable concentrations effectively inhibited the growth of the catecholamine-positive neuroblastoma cell line SK-N-SH; it inhibited LS cells to a smaller extent and catecholamine-negative SK-N-LO cell growth least effectively. In all three cell lines high concentrations of H2O2 were found. Since ascorbic acid was shown to release iron from ferritin in vitro and to keep it in the reduced state, we suggested that it acted as a pro-oxidant in ferritin-rich neuroblastoma cells in the presence of H2O2 and Fe2+ (Fenton reaction), implying iron release from cellular ferritin. We show here that iron could be mobilized from cellular ferritin by 1 mM ascorbic acid in iron-59-preloaded SK-N-SH and LS cells, but not in SK-N-LO cells. In agreement with these results, DNA strand break formation by ascorbate was only observed in SK-N-SH and LS cells. In SK-N-LO cells, DNA strand breaks could be induced by a combination of 1 mM ascorbic acid and 100 microM H2O2. Since cell-damaging effects caused by chemotherapy further facilitate iron release from ferritin, we conclude that ascorbate could be a powerful enhancer of some cytostatic drugs in neuroblastoma therapy.
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