ArticleInfection and immunity1994
Uroepithelial cells are part of a mucosal cytokine network.
Article in Infection and immunity, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 100 citations in OpenAlex.
- Prophylactic Potential ofMicroorganisms · 2026Article
- Effect of pro-inflammatory cytokines on urothelial cell adenosine triphosphate release and breakdown.Bladder (San Francisco, Calif.) · 2024Article
- Effect of Mannan Oligosaccharides Extracts in UropathogenicPathogens (Basel, Switzerland) · 2023Article
- Anti-inflammatory effects of lavender and eucalyptus essential oils on the in vitro cell culture model of bladder pain syndrome using T24 cells.BMC complementary medicine and therapies · 2022Article
- A prospective observational study of urinary cytokines and inflammatory response in patients with Overactive Bladder Syndrome.BMC urology · 2021Observational
- Interleukin-6/Stat3 signaling has an essential role in the host antimicrobial response to urinary tract infection.Kidney international · 2018Article
- Structural basis for IL-1α recognition by a modified DNA aptamer that specifically inhibits IL-1α signaling.Nature communications · 2017Article
- Article
- NKG2D is a Key Receptor for Recognition of Bladder Cancer Cells by IL-2-Activated NK Cells and BCG Promotes NK Cell Activation.Frontiers in immunology · 2015Article
- Effect of ceftriaxone on the outcome of murine pyelonephritis caused by extended-spectrum-β-lactamase-producing Escherichia coli.Antimicrobial agents and chemotherapy · 2014Article
- Article
- Assessment of urine solute and matrix effects on the performance of an enzyme-linked immunosorbent assay for measurement of interleukin-6 in dog urine.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2011Article
- Autocrine effects of interleukin-6 mediate acute-phase proinflammatory and tissue-reparative transcriptional responses of canine bladder mucosa.Infection and immunity · 2011Article
- The IL-6 -174G/C polymorphism and renal scarring in children with first acute pyelonephritis.Pediatric nephrology (Berlin, Germany) · 2010Article
- Host-pathogen interactions in urinary tract infection.Nature reviews. Urology · 2010Review
- Waging war against uropathogenic Escherichia coli: winning back the urinary tract.Infection and immunity · 2010Review
- G-CSF induction early in uropathogenic Escherichia coli infection of the urinary tract modulates host immunity.Cellular microbiology · 2008Article
- Identification of the mycobacterial subcomponents involved in the release of tumor necrosis factor-related apoptosis-inducing ligand from human neutrophils.Infection and immunity · 2007Article
- Neutrophils and TRAIL: insights into BCG immunotherapy for bladder cancer.Immunologic research · 2007Review
- Candida-induced oral epithelial cell responses.Mycopathologia · 2006Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study compared the cytokine production of uroepithelial cell lines in response to gram-negative bacteria and inflammatory cytokines. Human kidney (A498) and bladder (J82) epithelial cell lines were stimulated with either Escherichia coli Hu734, interleukin 1 alpha (IL-1 alpha), or tumor necrosis factor alpha (TNF-alpha). Supernatant samples were removed, and the RNA was extracted from cells at 0, 2, 6, and 24 h. The secreted cytokine levels were determined by bioassay or immunoassay; mRNA was examined by reverse transcription-PCR. The two cell lines secreted IL-6 and IL-8 constitutively. IL-6 and IL-8 mRNA were constitutively produced in both cell lines; IL-1 beta mRNA was detected in J82 cells. IL-1 alpha induced significantly higher levels of IL-6 secretion than did E. coli Hu734 or TNF-alpha. IL-1 alpha and TNF-alpha induced significantly higher levels of IL-8 secretion than did E. coli Hu734. Secreted IL-1 beta was not detected; IL-1 alpha and TNF-alpha were not detected above the levels used for stimulation. IL-1 alpha, IL-1 beta, IL-6, and IL-8 mRNAs were detected in both cell lines after exposure to the stimulants. TNF-alpha mRNA was occasionally detected in the J82 cell line after TNF-alpha stimulation. Cytokine (IL-6 and IL-8) and control (glyceraldehyde 3-phosphate dehydrogenase [G3PDH] and beta-actin) mRNA concentrations were quantitated with internal PCR standards. Cytokine mRNA levels relative to beta-actin mRNA levels were the highest in E. coli-stimulated cells. In comparison, the cytokine mRNA levels relative to G3PDH mRNA levels were the highest in IL-1 alpha-stimulated cells. beta-Actin mRNA levels decreased after bacterial stimulation but not after cytokine stimulation, while G3PDH mRNA levels increased in response to all of the stimulants tested. These results suggested that E. coli Hu734 lowered the beta-actin mRNA levels in uroepithelial cells, thus distorting the IL-6 and IL-8 mRNA levels relative to this control. In summary, E. coli IL-1 alpha and TNF-alpha were found to activate the de novo synthesis and secretion of IL-6 and IL-8 in uroepithelial cells. These results emphasize the role of epithelial cells in cytokine-mediated responses during the early stages of infection.
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Registered trials
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