ArticleInfection and immunity1994
Attachment of Cryptosporidium parvum sporozoites to MDCK cells in vitro.
Article in Infection and immunity, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 46 citations in OpenAlex.
- The role of atypical MAP kinase 4 in the host interaction with Cryptosporidium parvum.Scientific reports · 2023Article
- Elongation factor-1α is a novel protein associated with host cell invasion and a potential protective antigen of Cryptosporidium parvum.The Journal of biological chemistry · 2013Article
- The cell biology of cryptosporidium infection.Microbes and infection · 2011Review
- The terminal sialic acid of glycoconjugates on the surface of intestinal epithelial cells activates excystation of Cryptosporidium parvum.Infection and immunity · 2008Article
- Microbial adhesion of Cryptosporidium parvum sporozoites: purification of an inhibitory lipid from bovine mucosa.The Journal of parasitology · 2004Article
- In vitro culture of Cryptosporidium muris in a human stomach adenocarcinoma cell line.The Korean journal of parasitology · 2004Article
- Efficacy of monoclonal antibodies against defined antigens for passive immunotherapy of chronic gastrointestinal cryptosporidiosis.Antimicrobial agents and chemotherapy · 2002Article
- Characterization of an intestinal epithelial cell receptor recognized by the Cryptosporidium parvum sporozoite ligand CSL.Infection and immunity · 2001Article
- Mediation of Cryptosporidium parvum infection in vitro by mucin-like glycoproteins defined by a neutralizing monoclonal antibody.Infection and immunity · 2000Article
- Molecular cloning and expression of a gene encoding Cryptosporidium parvum glycoproteins gp40 and gp15.Infection and immunity · 2000Article
- Heterologous expression of Trypanosoma cruzi trans-sialidase in Leishmania major enhances virulence.Infection and immunity · 2000Article
- Intestinal epithelial cell apoptosis following Cryptosporidium parvum infection.Infection and immunity · 2000Article
- Human intestinal and biliary cryptosporidiosis.World journal of gastroenterology · 1999Article
- Attachment of Cryptosporidium parvum sporozoites to human intestinal epithelial cells.Infection and immunity · 1998Article
- Attachment of Toxoplasma gondii to host cells is host cell cycle dependent.Infection and immunity · 1996Article
- Complete development of Cryptosporidium parvum in bovine fallopian tube epithelial cells.Infection and immunity · 1996Article
- Effects of select medium supplements on in vitro development of Cryptosporidium parvum in HCT-8 cells.Journal of clinical microbiology · 1995Article
- Cryptosporidium parvum infection of Caco-2 cell monolayers induces an apical monolayer defect, selectively increases transmonolayer permeability, and causes epithelial cell death.Infection and immunity · 1994Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The initial attachment of Cryptosporidium parvum sporozoites to host cells in vivo may be a critical event in the pathogenesis of this infection. The molecular basis of attachment and the conditions influencing this host-parasite interaction have not been studied systematically. Therefore, we have developed a sporozoite attachment model by using paraformaldehyde-fixed Madin-Darby canine kidney (MDCK) cells. Attachment of sporozoites to fixed MDCK cells was quantitated by indirect immunofluorescence and confirmed by transmission electron microscopy. Attachment in this system was time, temperature (37 degrees C), and pH (7.2 to 7.6) dependent. Dose-response studies demonstrated that the attachment of sporozoites to fixed MDCK cells was a saturable process. Attachment was enhanced in the presence of 10 mM manganese, 1 mM calcium, and 1 to 10 mM zinc. Attachment of sporozoites to MDCK cells was inhibited in a dose-dependent manner by polyclonal anti-Cryptosporidium antisera and by purified immunoglobulin G (IgG). This model will be useful for the study of parasite and host cell molecules involved in the initial interaction of C. parvum sporozoites with their target cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.