ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology1993
Polymerase chain reaction for detection of Mycobacterium tuberculosis in sputum.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it.
- In-house nucleic acid amplification tests for the detection of Mycobacterium tuberculosis in sputum specimens: meta-analysis and meta-regression.BMC microbiology · 2005Pooled it
- Diagnostic value of an amplification method (Gen-Probe) compared with that of culture for diagnosis of tuberculosis.Journal of clinical microbiology · 1995Trial
- Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by Amplicor PCR.Journal of clinical microbiology · 1995Trial
- Use of the hupB gene encoding a histone-like protein of Mycobacterium tuberculosis as a target for detection and differentiation of M. tuberculosis and M. bovis.Journal of clinical microbiology · 2004Article
- Molecular diagnosis of Chlamydia pneumoniae infection.Journal of clinical microbiology · 1999Review
- Diagnostic use of PCR for detection of Pneumocystis carinii in oral wash samples.Journal of clinical microbiology · 1998Article
- Method for reduction of inhibition in a Mycobacterium tuberculosis-specific ligase chain reaction DNA amplification assay.Journal of clinical microbiology · 1998Article
- Relevance of nucleic acid amplification techniques for diagnosis of respiratory tract infections in the clinical laboratory.Clinical microbiology reviews · 1997Review
- Specificity of IS6110-based amplification assays for Mycobacterium tuberculosis complex.Journal of clinical microbiology · 1996Article
- Evaluation of mtp40 genomic fragment amplification for specific detection of Mycobacterium tuberculosis in clinical specimens.Journal of clinical microbiology · 1996Article
- Comparison of Amplicor, in-house PCR, and conventional culture for detection of Mycobacterium tuberculosis in clinical samples.Journal of clinical microbiology · 1995Article
- Routine application of the polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples.Journal of clinical pathology · 1995Article
- Article
- Evaluation of a commercial rRNA target amplification assay for detection of Mycobacterium tuberculosis complex in respiratory specimens.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 1994Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The polymerase chain reaction (PCR) was evaluated in a trial which, with respect to the positive-to-negative ratio, approximated the situation of a diagnostic laboratory in a tuberculosis-endemic area. Three hundred sputum samples were included in the study, of which one-third were known to contain mycobacteria as judged by direct microscopy. The repetitive insertion sequence IS6110/IS986 of Mycobacterium tuberculosis was used as a target. The samples were spiked with DNA from a modified IS6110/IS986 sequence, which gives rise to PCR products easily distinguished from PCR products amplified from chromosomal Mycobacterium tuberculosis DNA. This allowed identification of samples that contained substances inhibitory to the Taq polymerase. The detection limit of the assay was 0.05 pg to 0.5 pg of purified Mycobacterium tuberculosis DNA, corresponding to 10 to 100 organisms. The sensitivity and specificity of the PCR was compared with that of conventional microscopy and culture. It was concluded that this method is fast and sensitive, but that culture currently is crucial for assessing viability and thus infectivity.
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