ArticleVirchows Archiv : an international journal of pathology1994
Immunohistochemical identification of Ito cells and their myofibroblastic transformation in adult human liver.
Article in Virchows Archiv : an international journal of pathology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 118 citations in OpenAlex.
- The postbiotic ReFerm® versus standard nutritional support in advanced alcohol-related liver disease (GALA-POSTBIO): a randomized controlled phase 2 trial.Nature communications · 2025Trial
- Pericytes in tissue fibrosis.American journal of physiology. Cell physiology · 2025Review
- A Case of Canine Hepatitis with Hepatocellular Attack by Non-Neoplastic Perforin-Laden Lymphocytes.Veterinary sciences · 2025Article
- Expression of 6 Biomarkers in Liver Grafts After Pediatric Liver Transplantation: Correlations with Histology, Biochemistry, and Outcome.Annals of transplantation · 2020Article
- A Bioreactor Technology for Modeling Fibrosis in Human and Rodent Precision-Cut Liver Slices.Hepatology (Baltimore, Md.) · 2019Article
- A novel technique to prepare a single cell suspension of isolated quiescent human hepatic stellate cells.Scientific reports · 2019Article
- Hepatic stellate cells derived from the nestin-positive cells in septum transversum during rat liver development.Medical molecular morphology · 2018Article
- Human hepatic stellate cell isolation and characterization.Journal of gastroenterology · 2018Review
- Immunofluorescence identifies distinct subsets of endothelial cells in the human liver.Scientific reports · 2017Article
- The role of immune cells in metabolism-related liver inflammation and development of non-alcoholic steatohepatitis (NASH).Reviews in endocrine & metabolic disorders · 2016Review
- Inhibition of liver fibrosis using vitamin A-coupled liposomes to deliver matrix metalloproteinase-2 siRNA in vitro.Molecular medicine reports · 2015Article
- Pigment epithelium-derived factor is an intrinsic antifibrosis factor targeting hepatic stellate cells.The American journal of pathology · 2010Article
- Development of Fibrosis in Nonalcoholic Steatosis through Combination of a Synthetic Diet Rich in Disaccharide and Low-Dose Lipopolysaccharides in the Livers of Zucker (fa/fa) Rats.Journal of clinical biochemistry and nutrition · 2009Article
- Insoluble, speckled cytosolic distribution of retinoic acid receptor alpha protein as a marker of hepatic stellate cell activation in vitro.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2009Article
- Vinculin and cellular retinol-binding protein-1 are markers for quiescent and activated hepatic stellate cells in formalin-fixed paraffin embedded human liver.Histochemistry and cell biology · 2009Article
- Dietary olive oil prevents carbon tetrachloride-induced hepatic fibrosis in mice.Journal of gastroenterology · 2009Article
- Stellate cell contraction: role, regulation, and potential therapeutic target.Clinics in liver disease · 2008Review
- Pegylated interferon-alpha plus taurine in treatment of rat liver fibrosis.World journal of gastroenterology · 2007Article
- Primary hepatic lymphoma with spindle cell components: a case report.Virchows Archiv : an international journal of pathology · 2006Review
- Increased immunoreactivities against endothelin-converting enzyme-1 and monocyte chemotactic protein-1 in hepatic stellate cells of rat fibrous liver induced by thioacetamide.Medical molecular morphology · 2005Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To identify Ito cells in normal and pathological adult human livers, immunohistochemical studies were performed by the avidin-biotin-peroxidase complex method using monoclonal antibodies for alpha-smooth muscle actin (ASMA), desmin, and vimentin. Fifty one needle biopsies, 7 surgically resected specimens, and 5 autopsy specimens were studied. In the normal adult liver vascular smooth muscle cells and pericytes, together with perisinusoidal cells with thin cytoplasmic processes were positive for ASMA. These latter cells formed a loose and discontinuous layer along the sinusoidal walls. Immunoelectron microscopy showed that the ASMA-positive perisinusoidal cells were Ito cells containing fat droplets. The other sinusoidal lining cells were negative for ASMA. In chronic liver disease, ASMA-positive Ito cells showed an increase in number, size, and the intensity of immunostaining in areas of piece-meal necrosis), and formed a continuous cellular network. These cells were dendritic in shape with irregularly elongated cytoplasmic processes and contained an increased amount of microfilaments, in association with loss of the characteristic fat droplets. Thus, their ultrastructural features corresponded to those of myofibroblastic cells. Ito cells showed no staining for desmin in both normal and pathological livers. These results indicate that immunohistochemistry using an anti-ASMA antibody is a sensitive and reliable method for the identification of both normal and transformed Ito cells in adult human livers.
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