ArticleThe Clinical investigator1994
Myofibrillogenesis in primary tissue cultures of adult human skeletal muscle: expression of desmin, titin, and nebulin.
Article in The Clinical investigator, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 27 citations in OpenAlex.
- Autologous myoblast transplantation for oculopharyngeal muscular dystrophy: a phase I/IIa clinical study.Molecular therapy : the journal of the American Society of Gene Therapy · 2014Trial
- Neuronal Agrin Promotes Proliferation of Primary Human Myoblasts in an Age-Dependent Manner.International journal of molecular sciences · 2022Article
- Superoxide Anion Production and Bioenergetic Profile in Young and Elderly Human Primary Myoblasts.Oxidative medicine and cellular longevity · 2018Article
- Low Intensity Exercise Training Improves Skeletal Muscle Regeneration Potential.Frontiers in physiology · 2015Article
- The role of Pitx2 in maintaining the phenotype of myogenic precursor cells in the extraocular muscles.PloS one · 2013Article
- Muscle giants: molecular scaffolds in sarcomerogenesis.Physiological reviews · 2009Review
- The identification of myogenic cells in skeletal muscle, with emphasis on the use of tritiated thymidine autoradiography and desmin antibodies.Journal of anatomy · 1998Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the in vitro development of myofibrils in skeletal muscle cells derived from adult human muscle biopsies, immunohistochemical analysis was performed using monoclonal antibodies against desmin, titin, and nebulin. Diffuse desmin reactivity was detected 48 h after plating in about 60% of all mononucleated cells. This supports the use of desmin as a marker for undifferentiated rhabdomyosarcomas in man. Titin was visible from day 4 onwards, while nebulin was not found in mononucleated cells. After 1 week polynucleated myotubes appeared, and grew up to 30 days. Desmin was distributed diffusely throughout the cytoplasm until day 21, when the pattern became patchy. Titin began to be organized in a predominantly longitudinal orientation at day 15, while nebulin, which appeared for the first time in fusing myoblasts on the fifth to the seventh day, was almost immediately organized in a dotted longitudinal pattern, which became a Z line connected striation in matured myotubes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.