ArticleMolecular biology of the cell1994
Systematic mutational analysis of the yeast beta-tubulin gene.
Article in Molecular biology of the cell, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 104 citations in OpenAlex.
- Role of α-tubulin helix 11' in heterodimer conformation and microtubule dynamics.bioRxiv : the preprint server for biology · 2026Article
- Arginylation of ⍺-tubulin at E77 regulates microtubule dynamics via MAP1S.The Journal of cell biology · 2025Article
- Structural insights into the mechanism of GTP initiation of microtubule assembly.Nature communications · 2023Article
- Exploring Pyrrolo-Fused Heterocycles as Promising Anticancer Agents: An Integrated Synthetic, Biological, and Computational Approach.Pharmaceuticals (Basel, Switzerland) · 2023Article
- The tubulin database: Linking mutations, modifications, ligands and local interactions.PloS one · 2023Article
- Interactions of Caenorhabditis elegans β-tubulins with the microtubule inhibitor and anthelmintic drug albendazole.Genetics · 2022Article
- Dual Impact of a Benzimidazole Resistant β-Tubulin on Microtubule Behavior in Fission Yeast.Cells · 2021Article
- Epistatic, synthetic, and balancing interactions among tubulin missense mutations affecting neurite growth inMolecular biology of the cell · 2021Article
- Structural and functional differences between porcine brain and budding yeast microtubules.Cell cycle (Georgetown, Tex.) · 2018Article
- Distinct effects of tubulin isotype mutations on neurite growth inMolecular biology of the cell · 2017Article
- Structural differences between yeast and mammalian microtubules revealed by cryo-EM.The Journal of cell biology · 2017Article
- Coordination of Cell Cycle Progression and Mitotic Spindle Assembly Involves Histone H3 Lysine 4 Methylation by Set1/COMPASS.Genetics · 2017Article
- Article
- Structural maintenance of chromosome (SMC) proteins link microtubule stability to genome integrity.The Journal of biological chemistry · 2014Article
- Structure-function analysis of yeast tubulin.Methods in cell biology · 2013Article
- Article
- α-Tubulin mutations alter oryzalin affinity and microtubule assembly properties to confer dinitroaniline resistance.Eukaryotic cell · 2010Article
- Mutation of Ser172 in yeast β tubulin induces defects in microtubule dynamics and cell division.PloS one · 2010Article
- B-cyclin/CDKs regulate mitotic spindle assembly by phosphorylating kinesins-5 in budding yeast.PLoS genetics · 2010Article
- Human TUBB3 mutations perturb microtubule dynamics, kinesin interactions, and axon guidance.Cell · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A systematic strategy was used to create a synoptic set of mutations that are distributed throughout the single beta-tubulin gene of Saccharomyces cerevisiae. Clusters of charged amino acids were targeted for mutagenesis and converted to alanine to maximize alterations on the protein's surface and minimize alterations that affect protein folding. Of the 55 mutations we constructed, three confer dominant-lethality, 11 confer recessive-lethality, 10 confer cold-sensitivity, one confers heat-sensitivity, and 27 confer altered resistance to benomyl. Only 11 alleles give no discernible phenotype. In spite of the fact that beta-tubulin is a highly conserved protein, three-fourths of the mutations do not destroy the ability of the protein to support the growth of yeast at 30 degrees C. The lethal substitutions are primarily located in three regions of the protein and presumably identify domains most critical for beta-tubulin function. Interestingly, most of the conditional-lethal alleles produce specific defects in spindle assembly at their restrictive temperature; cytoplasmic microtubules are relatively unaffected. The exceptions are two mutants that contain abnormally long cytoplasmic microtubules. Mutants with specific spindle defects were not observed in our previous collection of beta-tubulin mutants and should be valuable in dissecting spindle function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.