ArticleThe Journal of membrane biology1994
Regulation of the gating of the sheep cardiac sarcoplasmic reticulum Ca(2+)-release channel by luminal Ca2+.
Article in The Journal of membrane biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
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Who cites it
91 citing papers in PubMed, 172 citations in OpenAlex.
- Review
- Novel Ca2+ wave mechanisms in cardiac myocytes revealed by multiscale Ca2+ release model.The Journal of general physiology · 2025Article
- The biophysical properties of TRIC-A and TRIC-B and their interactions with RyR2.The Journal of general physiology · 2023Article
- Mechanisms of spontaneous CaThe Journal of physiology · 2023Article
- Avian cardiomyocyte architecture and what it reveals about the evolution of the vertebrate heart.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Biological noise is a key determinant of the reproducibility and adaptability of cardiac pacemaking and EC coupling.The Journal of general physiology · 2022Review
- The V2475F CPVT1 mutation yields distinct RyR2 channel populations that differ in their responses to cytosolic CaThe Journal of physiology · 2021Article
- Understanding How Phosphorylation and Redox Modifications Regulate Cardiac Ryanodine Receptor Type 2 Activity to Produce an Arrhythmogenic Phenotype in Advanced Heart Failure.ACS pharmacology & translational science · 2020Review
- Paxilline Prevents the Onset of Myotonic Stiffness in Pharmacologically Induced Myotonia: A Preclinical Investigation.Frontiers in physiology · 2020Article
- CaCells · 2019Review
- Ion channel gating in cardiac ryanodine receptors from the arrhythmic RyR2-P2328S mouse.Journal of cell science · 2019Article
- Unbalance Between Sarcoplasmic Reticulum CaFrontiers in physiology · 2019Review
- Regulation of the RyR channel gating by CaBiophysical reviews · 2018Review
- Proarrhythmic Remodeling of Calcium Homeostasis in Cardiac Disease; Implications for Diabetes and Obesity.Frontiers in physiology · 2018Review
- The structural basis of ryanodine receptor ion channel function.The Journal of general physiology · 2017Review
- The role of luminal Ca regulation in Ca signaling refractoriness and cardiac arrhythmogenesis.The Journal of general physiology · 2017Review
- Quenching the spark: Termination of CICR in the submicroscopic space of the dyad.The Journal of general physiology · 2017Review
- Calcium and Excitation-Contraction Coupling in the Heart.Circulation research · 2017Review
- Murine Electrophysiological Models of Cardiac Arrhythmogenesis.Physiological reviews · 2017Review
- Cytosolic Ca²⁺ buffering determines the intra-SR Ca²⁺ concentration at which cardiac Ca²⁺ sparks terminate.Cell calcium · 2015Article
31 more citing papers are in PubMed but not listed here.
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2 authors.
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Abstract
We investigated the effects of changes in luminal [Ca2+] on the gating of native and purified sheep cardiac sarcoplasmic reticulum (SR) Ca(2+)-release channels reconstituted into planar phospholipid bilayers. The open probability (Po) of channels activated solely by cytosolic Ca2+ was greater at positive than negative holding potentials. Channels activated solely by 10 microM cytosolic Ca2+ exhibited no change in steady-state Po or in the relationship between Po and voltage when the luminal [Ca2+] was increased from nanomolar to millimolar concentrations. In the absence of activating concentrations of cytosolic Ca2+, the channel can be activated by the phosphodiesterase inhibitor sulmazole (AR-L 115BS). However, cytosolic Ca(2+)-independent activation of the channel by sulmazole requires luminal Ca2+. In the presence of sulmazole, at picomolar luminal [Ca2+] the channel remains completely closed. Increasing the luminal [Ca2+] to millimolar levels markedly increases the Po via an increase in the duration of open events. The Po and duration of the sulmazole-activated, luminal Ca(2+)-dependent channel openings are voltage dependent. In the presence of micromolar luminal Ca2+, the Po and duration of sulmazole-activated openings are greater at negative voltages. However, at millimolar luminal [Ca2+], long openings are also observed at positive voltages and the Po appears to be similar at positive and negative voltages. Our findings indicate that the regulation of channel gating by luminal Ca2+ depends on the mechanism of channel activation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.