ArticleMolecular & general genetics : MGG1994
The yeast co-activator GAL11 positively influences transcription of the phosphoglycerate kinase gene, but only when RAP1 is bound to its upstream activation sequence.
Article in Molecular & general genetics : MGG, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed, 17 citations in OpenAlex.
- Mutations in GCR1 affect SUC2 gene expression in Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2003Article
- Different roles for abf1p and a T-rich promoter element in nucleosome organization of the yeast RPS28A gene.Nucleic acids research · 2000Article
- Multiple domains of repressor activator protein 1 contribute to facilitated binding of glycolysis regulatory protein 1.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Activation mechanism of the multifunctional transcription factor repressor-activator protein 1 (Rap1p).Molecular and cellular biology · 1996Article
- The 3-phosphoglycerate kinase gene of the yeast Yarrowia lipolytica de-represses on gluconeogenic substrates.Current genetics · 1996Article
- Control of glycolytic gene expression in the budding yeast (Saccharomyces cerevisiae).Current genetics · 1995Review
- The yeast protein Gcr1p binds to the PGK UAS and contributes to the activation of transcription of the PGK gene.Molecular & general genetics : MGG · 1994Article
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5 authors at 3 institutions in 2 countries.
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Abstract
Transcription of the yeast phosphoglycerate kinase gene (PGK) is activated by an array of nuclear factors including the multifunctional protein RAP1. We have demonstrated that the transcriptional co-activator GAL11, which was identified as an auxiliary factor to GAL4 and which is believed to interact with the zinc finger of the trans-activator, positively influences the level of PGK transcription on both fermentable and non-fermentable carbon sources. This positive effect is only observed when the RAP1 site in the upstream activation sequence (UAS) is present, implying that GAL11 acts through RAP1. Expression of the RAP1 gene is not reduced in the gal11 background, and in vivo footprinting shows that GAL11 does not influence RAP1 DNA-binding activity. Therefore the effect of GAL11 on PGK transcription must be mediated at the PGK UAS, presumably as part of the activation complex. It has been proposed that RAP1 may act as a facilitator of GCR1 binding at the PGK UAS and therefore it is conceivable that the target for GAL11 may in fact be GCR1. A further implication of this study is that GAL11 can interact with proteins such as RAP1 or GCR1 that are apparently structurally dissimilar from GAL4 and other zinc finger DNA-binding proteins.
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