ArticleThe Journal of cell biology1994
CENP-C is required for maintaining proper kinetochore size and for a timely transition to anaphase.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
78 citing papers in PubMed, 203 citations in OpenAlex.
- CCAN forms the spherical shell-like structure in interphase kinetochores.Science advances · 2026Article
- Low kinetochore-microtubule occupancy leaves a mitotic memory by delaying checkpoint silencing.Nature communications · 2026Article
- The dynamic centromere.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- CENP-C-Mis12 complex establishes a regulatory loop through Aurora B for chromosome segregation.Life science alliance · 2025Article
- An evolutionary perspective on the relationship between kinetochore size and CENP-E dependence for chromosome alignment.Journal of cell science · 2024Article
- Determinants of minor satellite RNA function in chromosome segregation in mouse embryonic stem cells.The Journal of cell biology · 2024Article
- Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution.Frontiers in cell and developmental biology · 2022Review
- Immunization with CENP-C Causes Aberrant Chromosome Segregation during Oocyte Meiosis in Mice.Journal of immunology research · 2021Article
- Centromeres under Pressure: Evolutionary Innovation in Conflict with Conserved Function.Genes · 2020Review
- Review
- Chromosome Segregation Is Biased by Kinetochore Size.Current biology : CB · 2018Article
- Stepwise unfolding supports a subunit model for vertebrate kinetochores.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- ATRX contributes to epigenetic asymmetry and silencing of major satellite transcripts in the maternal genome of the mouse embryo.Development (Cambridge, England) · 2015Article
- The dimerization domain of PfCENP-C is required for its functions as a centromere protein in human malaria parasite Plasmodium falciparum.Malaria journal · 2014Article
- Lessons learned from counting molecules: how to lure CENP-A into the kinetochore.Open biology · 2014Article
- The architecture of CCAN proteins creates a structural integrity to resist spindle forces and achieve proper Intrakinetochore stretch.Developmental cell · 2014Article
- HACking the centromere chromatin code: insights from human artificial chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Review
- Epigenetic engineering: histone H3K9 acetylation is compatible with kinetochore structure and function.Journal of cell science · 2012Article
- Article
- The ABCs of CENPs.Chromosoma · 2011Review
18 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The human autoantigen CENP-C has been demonstrated by immunoelectron microscopy to be a component of the inner kinetochore plate. Here we have used antibodies raised against various portions of CENP-C to probe its function in mitosis. We show that nuclear microinjection of anti-CENP-C antibodies during interphase causes a transient arrest at the following metaphase. Injection of the same antibodies after the initiation of prophase, however, does not disrupt mitosis. Correspondingly, indirect immunofluorescence using affinity-purified human anti-CENP-C antibodies reveals that levels of CENP-C staining are reduced at centromeres in cells that were injected during interphase, but appear unaffected in cells which were injected during mitosis. Thus, we suggest that the injected antibodies cause metaphase arrest by reducing the amount of CENP-C at centromeres. Examination of kinetochores in metaphase-arrested cells by electron microscopy reveals that the number of trilaminar structures is reduced. More surprisingly, the few remaining kinetochores in these cells retain a normal trilaminar morphology but are significantly reduced in diameter. In cells arrested for extended periods, these small kinetochores become disrupted and apparently no longer bind microtubules. These observations are consistent with an involvement of CENP-C in kinetochore assembly, and suggest that CENP-C plays a critical role in both establishing and/or maintaining proper kinetochore size and stabilizing microtubule attachments. These findings also support the idea that proper assembly of kinetochores may be monitored by the cell cycle checkpoint preceding the transition to anaphase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.