ArticleThe Biochemical journal1994
Subcellular localization and translocation of the receptor for N-formylmethionyl-leucyl-phenylalanine in human neutrophils.
Article in The Biochemical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 145 citations in OpenAlex.
- Galectin-9 signaling through TIM-3 is involved in neutrophil-mediated Gram-negative bacterial killing: an effect abrogated within the cystic fibrosis lung.Journal of immunology (Baltimore, Md. : 1950) · 2014Trial
- Parallel CRISPR screens reveal pathways controlling the cell surface levels of the attractant receptor FPR1.Communications biology · 2026Article
- Neutrophil diversity and function in health and disease.Signal transduction and targeted therapy · 2024Review
- Increased expression of formyl peptide receptor-1 by basophils from patients with mastocytosis.The journal of allergy and clinical immunology. Global · 2024Article
- ATG5 provides host protection acting as a switch in the atg8ylation cascade between autophagy and secretion.Developmental cell · 2023Article
- Therapeutic assessment of N-formyl-methionyl-leucyl-phenylalanine (fMLP) in reducing periprosthetic joint infection.European cells & materials · 2021Article
- Mitochondrial N-formyl methionine peptides associate with disease activity as well as contribute to neutrophil activation in patients with rheumatoid arthritis.Journal of autoimmunity · 2021Article
- Article
- Vesicular nucleotide transporter mediates ATP release and migration in neutrophils.The Journal of biological chemistry · 2018Article
- Use of a leukocyte-targeted peptide probe as a potential tracer for imaging the tuberculosis granuloma.Tuberculosis (Edinburgh, Scotland) · 2018Article
- The Neutrophil Response Induced by an Agonist for Free Fatty Acid Receptor 2 (GPR43) Is Primed by Tumor Necrosis Factor Alpha and by Receptor Uncoupling from the Cytoskeleton but Attenuated by Tissue Recruitment.Molecular and cellular biology · 2016Article
- The role of formylated peptides and formyl peptide receptor 1 in governing neutrophil function during acute inflammation.The American journal of pathology · 2015Review
- Targeting pancreatic cancer with magneto-fluorescent theranostic gold nanoshells.Nanomedicine (London, England) · 2014Article
- The subcellular localization of the receptor for platelet-activating factor in neutrophils affects signaling and activation characteristics.Clinical & developmental immunology · 2013Article
- The multifaceted roles of neutrophil gelatinase associated lipocalin (NGAL) in inflammation and cancer.Biochimica et biophysica acta · 2012Review
- Neutrophils in innate host defense against Staphylococcus aureus infections.Seminars in immunopathology · 2012Review
- Endothelium-derived GM-CSF influences expression of oncostatin M.American journal of physiology. Cell physiology · 2011Article
- International Union of Basic and Clinical Pharmacology. LXXIII. Nomenclature for the formyl peptide receptor (FPR) family.Pharmacological reviews · 2009Review
- The role of lipocalin 2 in the regulation of inflammation in adipocytes and macrophages.Molecular endocrinology (Baltimore, Md.) · 2008Article
- Decreased serum lipocalin-2 levels in human immunodeficiency virus-infected patients: increase during highly active anti-retroviral therapy.Clinical and experimental immunology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The subcellular localization of N-formylmethionyl-leucyl-phenylalanine (fMLP) receptors in human neutrophils was investigated. The fMLP receptor was detected with a high-affinity, photoactivatable, radioiodinated derivative of N-formyl-methionyl-leucyl-phenylalanyl-lysine (fMLFK). Neutrophils were disrupted by nitrogen cavitation and fractionated on Percoll density gradients. fMLP receptors were located in the beta-band containing gelatinase and specific granules, and in the gamma-band containing plasma membrane and secretory vesicles. Plasma membranes and secretory vesicles were separated by high-voltage free-flow electrophoresis, and secretory vesicles were demonstrated to be highly enriched in fMLP receptors. The receptors found in secretory vesicles translocated fully to the plasma membrane upon stimulation with inflammatory mediators. The receptor translocation from the beta-band indicated that the receptor present there was mainly located in gelatinase granules. A 25 kDa fMLP-binding protein was found in the beta-band. Immunoprecipitation revealed that this protein was identical with NGAL (neutrophil gelatinase-associated lipocalin), a novel protein found in specific granules. In summary, we demonstrate that the compartment in human neutrophils that is mobilized most easily and fastest, the secretory vesicle, is a major reservoir of fMLP receptors. This explains the prompt and extensive upregulation of fMLP receptors on the neutrophil surface in response to inflammatory stimuli.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.