ArticleThe Journal of general physiology1994
Regulation of cAMP-activated apical membrane chloride conductance in gallbladder epithelium.
Article in The Journal of general physiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- Effects of Eimeria separata infections on Na+ and Cl- transport in the rat large intestine.Parasitology research · 2004Article
- PKC-mediated stimulation of amphibian CFTR depends on a single phosphorylation consensus site. insertion of this site confers PKC sensitivity to human CFTR.The Journal of general physiology · 2001Article
- A primary culture of guinea pig gallbladder epithelial cells that is responsive to secretagogues.American journal of physiology. Gastrointestinal and liver physiology · 2000Article
- Stretch-activated single K+ channels account for whole-cell currents elicited by swelling.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Cell swelling activates the K+ conductance and inhibits the Cl- conductance of the basolateral membrane of cells from a leaky epithelium.The Journal of general physiology · 1997Article
- Electrogenic cation transport across leech caecal epithelium.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 1996Article
- The Ca(2+)-induced leak current in Xenopus oocytes is indeed mediated through a Cl- channel.The Journal of membrane biology · 1995Article
- Effects of cyclic AMP and theophylline on chloride conductance across toad skin.The Journal of physiology · 1995Article
- Hydrochlorothiazide action on the apical Cl-, Ca2+ and K+ conductances in rabbit gallbladder epithelium. Presence of an apamin-sensitive, Ca(2+)-activated K+ conductance.The Journal of membrane biology · 1995Article
- Volume-activated chloride currents are not correlated with P-glycoprotein expression.The Biochemical journal · 1995Article
- Calcium is not involved in the cAMP-mediated stimulation of Cl- conductance in the apical membrane of Necturus gallbladder epithelium.Pflugers Archiv : European journal of physiology · 1995Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Regulation of the cAMP-activated apical membrane Cl- conductance (GaCl) in Necturus gallbladder (NGB) epithelial cells was investigated with intracellular-microelectrode techniques. GaCl was increased by exposure to 8-Br-cAMP, theophylline or forskolin. Neither 8-Br-cGMP nor elevation of intracellular [Ca2+] using ionomycin had effects on GaCl or interfered with activation of GaCl by forskolin. N-(2-[methylamino]ethyl)-5-isoquinolinesulfonamide (H8), an inhibitor of cAMP-dependent protein kinase (PKA), slowed but did not prevent the GaCl response to 8-Br-cAMP. Phorbol 12-myristate 13-acetate (PMA), which activates protein kinase C (PKC), stimulated GaCl but had no effects on intracellular [cAMP]. GaCl was unaffected by 4 alpha-phorbol, a PMA analog which does not activate PKC. Okadaic acid (OA), an inhibitor of protein phosphatases (PP) types 1 and 2A, slowed the activation of GaCl by 8-Br-cAMP, hastened the return of GaCl to basal values following removal of 8-Br-cAMP, and significantly reduced the elevation in intracellular [cAMP] produced by forskolin. OA had no effects on the GaCl changes elicited by theophylline. We conclude that: (a) NGB GaCl can be activated by PKA-mediated phosphorylation of apical membrane Cl- channels or a regulatory protein, (b) GaCl can also be activated via PKC, by a cAMP-independent mechanism, (c) OA-sensitive PP are not required for inactivation of GaCl; OA appears to stimulate phosphodiesterase, which lowers intracellular [cAMP] and affects GaCl activation, and (d) the apical membrane of NGB epithelium lacks a Ca(2+)-activated Cl- conductance.
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