ArticleInfection and immunity1994
Evidence for adhesin activity in the acid-stable moiety of the phosphomannoprotein cell wall complex of Candida albicans.
Article in Infection and immunity, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 69 citations in OpenAlex.
- Immunochemistry of pathogenic yeast, Candida species, focusing on mannan.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2012Review
- Article
- Horizontal transmission of Candida albicans and evidence of a vaccine response in mice colonized with the fungus.PloS one · 2011Article
- Concise assembly of linear alpha(1-->6)-linked octamannan fluorescent probe.Tetrahedron letters · 2008Article
- Chemical structure of the cell-wall mannan of Candida albicans serotype A and its difference in yeast and hyphal forms.The Biochemical journal · 2007Article
- Mnt1p and Mnt2p of Candida albicans are partially redundant alpha-1,2-mannosyltransferases that participate in O-linked mannosylation and are required for adhesion and virulence.The Journal of biological chemistry · 2005Article
- Surface glycans of Candida albicans and other pathogenic fungi: physiological roles, clinical uses, and experimental challenges.Clinical microbiology reviews · 2004Review
- Relative abundance of oligosaccharides in Candida species as determined by fluorophore-assisted carbohydrate electrophoresis.Journal of clinical microbiology · 2000Article
- Protection against candidiasis by an immunoglobulin G3 (IgG3) monoclonal antibody specific for the same mannotriose as an IgM protective antibody.Infection and immunity · 2000Article
- Minimum chemical requirements for adhesin activity of the acid-stable part of Candida albicans cell wall phosphomannoprotein complex.Infection and immunity · 1998Article
- Cell wall and secreted proteins of Candida albicans: identification, function, and expression.Microbiology and molecular biology reviews : MMBR · 1998Review
- Serologic response to cell wall mannoproteins and proteins of Candida albicans.Clinical microbiology reviews · 1998Review
- Protective role of antimannan and anti-aspartyl proteinase antibodies in an experimental model of Candida albicans vaginitis in rats.Infection and immunity · 1997Article
- Evidence for the presence of immunoglobulin E antibodies specific to the cell wall phosphomannoproteins of Candida albicans in patients with allergies.Clinical and diagnostic laboratory immunology · 1996Article
- Antigenicity of cell wall mannans of Candida albicans NIH B-792 (serotype B) strain cells cultured at high temperature in yeast extract-containing sabouraud liquid medium.Clinical and diagnostic laboratory immunology · 1996Article
- Adhesion of Candida albicans to brain tissue of Macaca mulata in an ex vivo assay.Infection and immunity · 1995Article
- Antibody response that protects against disseminated candidiasis.Infection and immunity · 1995Article
- Rats clearing a vaginal infection by Candida albicans acquire specific, antibody-mediated resistance to vaginal reinfection.Infection and immunity · 1995Article
- Beta-1,2-linked oligomannosides from Candida albicans act as signals for tumor necrosis factor alpha production.Infection and immunity · 1995Article
- Murine tissues exposed to cytotoxic drugs display altered patterns of Candida albicans adhesion.Infection and immunity · 1994Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Previously, we showed that Candida albicans hydrophilic yeast cells adhere specifically to mouse splenic marginal-zone macrophages. The adhesins are part of the yeast cell wall phosphomannoprotein complex, and one adhesin site, which reacts with the monoclonal antibody 10G, was identified as a beta-1,2-linked tetramannose in the acid-labile portion of the complex. We report here that the acid-stable part of the complex, which has not been reported previously to have adhesin activity, is in large part responsible for yeast cell binding to the splenic marginal zone. The phosphomannoprotein complex, termed Fr.II, was isolated from C. albicans serotype B yeast cells by beta-mercaptoethanol extraction and concanavalin A-agarose affinity chromatography. Fr.II is devoid of the serotype A-specific antigen factor 6, which functions in yeast cell attachment to epithelial cells. The acid-stable part of Fr.II (i.e., Fr.IIS) was obtained by mild acid hydrolysis and size exclusion fractionation. Fr.IIS was further fractionated into four fractions, Fr.IIS1, Fr.IIS2, Fr.IIS3, and Fr.IIS4, by concanavalin A-agarose column chromatography and elution with a linear gradient of alpha-methyl-D-mannopyranoside. Adhesin activity of these fractions was determined by their ability to block yeast cell binding to the splenic marginal zone. Fr.IIS1 and Fr.IIS2 yielded more material and stronger adhesin activity than either Fr.IIS3 or Fr.IIS4. Only Fr.IIS1 did not react with antibodies (anti-factor 5 and monoclonal antibody 10G) specific for the acid-labile beta-1,2-linked oligosaccharides. Fr.IIS1-coated latex beads attached specifically to the marginal zone in a pattern identical to that of yeast cell binding. Furthermore, Fr.IIS1-latex bead attachment was inhibited by soluble Fr.II or Fr.IIS. Initial chemical analyses indicate that the adhesin site on Fr.IIS1 is a carbohydrate because adhesin activity was destroyed by periodate oxidation but not by proteinase K digestion.
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