ArticleMolecular biology of the cell1993
Alteration of the Saccharomyces cerevisiae COX2 mRNA 5'-untranslated leader by mitochondrial gene replacement and functional interaction with the translational activator protein PET111.
Article in Molecular biology of the cell, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 73 citations in OpenAlex.
- Allotopic expression ofLife science alliance · 2023Article
- Atco, a yeast mitochondrial complex of Atp9 and Cox6, is an assembly intermediate of the ATP synthase.PloS one · 2020Article
- Yeast mitochondrial protein Pet111p binds directly to two distinct targets inThe Journal of biological chemistry · 2019Article
- Gcn5 histone acetyltransferase is present in the mitoplasts.Biology open · 2019Article
- Mitochondrial versus nuclear gene expression and membrane protein assembly: the case of subunit 2 of yeast cytochromeMolecular biology of the cell · 2018Article
- Novel mutation in mitochondrial Elongation Factor EF-Tu associated to dysplastic leukoencephalopathy and defective mitochondrial DNA translation.Biochimica et biophysica acta. Molecular basis of disease · 2017Article
- Strain-specific nuclear genetic background differentially affects mitochondria-related phenotypes in Saccharomyces cerevisiae.MicrobiologyOpen · 2014Article
- Review
- The Cox3p assembly module of yeast cytochrome oxidase.Molecular biology of the cell · 2014Article
- Yeast PPR proteins, watchdogs of mitochondrial gene expression.RNA biology · 2013Article
- Structural and functional role of bases 32 and 33 in the anticodon loop of yeast mitochondrial tRNAIle.RNA (New York, N.Y.) · 2011Article
- Peptides from aminoacyl-tRNA synthetases can cure the defects due to mutations in mt tRNA genes.Mitochondrion · 2011Article
- Deleterious effect of the Qo inhibitor compound resistance-conferring mutation G143A in the intron-containing cytochrome b gene and mechanisms for bypassing it.Applied and environmental microbiology · 2011Article
- Article
- Yeast as a model of human mitochondrial tRNA base substitutions: investigation of the molecular basis of respiratory defects.RNA (New York, N.Y.) · 2008Article
- Alteration of a novel dispensable mitochondrial ribosomal small-subunit protein, Rsm28p, allows translation of defective COX2 mRNAs.Eukaryotic cell · 2005Article
- Saccharomyces cerevisiae translational activator Cbs2p is associated with mitochondrial ribosomes.Current genetics · 2004Article
- MrpL36p, a highly diverged L31 ribosomal protein homolog with additional functional domains in Saccharomyces cerevisiae mitochondria.Genetics · 2004Article
- Expression of Barstar as a selectable marker in yeast mitochondria.Molecular genetics and genomics : MGG · 2003Article
- The Rieske FeS protein encoded and synthesized within mitochondria complements a deficiency in the nuclear gene.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
The ability to replace wild-type mitochondrial DNA sequences in yeast with in vitro-generated mutations has been exploited to study the mechanism by which the nuclearly encoded PET111 protein specifically activates translation of the mitochondrially coded COX2 mRNA. We have generated three mutations in vitro that alter the COX2 mRNA 5'-untranslated leader (UTL) and introduced them into the mitochondrial genome, replacing the wild-type sequence. None of the mutations significantly affected the steady-state level of COX2 mRNA. Deletion of a single base at position -24 (relative to the translation initiation codon) in the 5'-UTL (cox2-11) reduced COX2 mRNA translation and respiratory growth, whereas insertion of four bases in place of the deleted base (cox2-12) and deletion of bases -30 to -2 (cox2-13) completely blocked both. Six spontaneous nuclear mutations were selected as suppressors of the single-base 5'-UTL deletion, cox2-11. One of these mapped to PET111 and was shown to be a missense mutation that changed residue 652 from Ala to Thr. This suppressor, PET111-20, failed to suppress the 29-base deletion, cox2-13, but very weakly suppressed the insertion mutation, cox2-12. PET111-20 also enhanced translation of a partially functional COX2 mRNA with a wild-type 5'-UTL but a mutant initiation codon. Although overexpression of the wild-type PET111 protein caused weak suppression of the single-base deletion, cox2-11, the PET111-20 suppressor mutation did not function simply by increasing the level of the protein. These results demonstrate an intimate functional interaction between the translational activator protein and the mRNA 5'-UTL and suggest that they may interact directly.
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