ArticleMolecular biology of the cell1993
Actin structure and function: roles in mitochondrial organization and morphogenesis in budding yeast and identification of the phalloidin-binding site.
Article in Molecular biology of the cell, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
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101 citing papers in PubMed, 253 citations in OpenAlex.
- Experimental Detection Methods and Clinical Translational Challenges of Disulfidptosis in Cancer.Cells · 2026Review
- Caloric restriction enhances inheritance of wild-type mitochondrial DNA in Saccharomyces cerevisiae.Scientific reports · 2025Article
- Functional redundancy and formin-isoform independent localization of tropomyosin paralogs in Saccharomyces cerevisiae.PLoS genetics · 2025Article
- A yeast based assay establishes the pathogenicity of novel missense ACTA2 variants associated with aortic aneurysms.European journal of human genetics : EJHG · 2024Article
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- Mitochondria-actin cytoskeleton crosstalk in cell migration.Journal of cellular physiology · 2022Review
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- High-resolution cryo-EM structures of actin-bound myosin states reveal the mechanism of myosin force sensing.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Organization and dynamics of yeast mitochondrial nucleoids.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2017Review
- The L-type Ca(2+) channel facilitates abnormal metabolic activity in the cTnI-G203S mouse model of hypertrophic cardiomyopathy.The Journal of physiology · 2016Article
- The Effects of Disease Models of Nuclear Actin Polymerization on the Nucleus.Frontiers in physiology · 2016Article
- Mitochondrial dynamics and inheritance during cell division, development and disease.Nature reviews. Molecular cell biology · 2014Review
- Redistribution of mitochondria leads to bursts of ATP production during spontaneous mouse oocyte maturation.Journal of cellular physiology · 2010Article
- A quantitative assay for mitochondrial fusion using Renilla luciferase complementation.Mitochondrion · 2010Article
- Article
- Influence of phalloidin on the formation of actin filament branches by Arp2/3 complex.Biochemistry · 2008Article
- Mutations in the RAM network confer resistance to the thiol oxidant 4,4'-dipyridyl disulfide.Molecular genetics and genomics : MGG · 2008Article
- Oxidant resistance in a yeast mutant deficient in the Sit4 phosphatase.Current genetics · 2008Article
- The class V myosin motor protein, Myo2, plays a major role in mitochondrial motility in Saccharomyces cerevisiae.The Journal of cell biology · 2008Article
41 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To further elucidate the functions of actin in budding yeast and to relate actin structure to specific roles and interactions in vivo, we determined the phenotypes caused by 13 charged-to-alanine mutations isolated previously in the single Saccharomyces cerevisiae actin gene. Defects in actin organization, morphogenesis, budding pattern, chitin deposition, septation, nuclear segregation, and mitochondrial organization were observed. In wild-type cells, mitochondria were found to be aligned along actin cables. Many of the amino acid substitutions that had the most severe effects on mitochondrial organization are located under the myosin "footprint" on the actin monomer, suggesting that actin-myosin interactions might underlie mitochondrial organization in yeast. In addition, one mutant (act1-129; R177A, D179A) produced an actin that assembled into cables and patches that could be visualized by anti-actin immunofluorescence in situ and that assembled into microfilaments of normal appearance in vitro as judged by electron microscopy but which could not be labeled by rhodamine-phalloidin in situ or in vitro. Rhodamine-phalloidin could label actin filaments assembled from all of the other mutant actins, including one (act1-119; R116A, E117A, K118A) that is altered at a residue (E117) that can be chemically cross-linked to phalloidin. The implication of residues R177 and/or D179 in phalloidin binding is in close agreement with a recently reported molecular model in which the phalloidin-binding site is proposed to be at the junction of two or three actin monomers in the filament.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.