ArticleMolecular biology of the cell1993
Determination of the functional domains involved in nucleolar targeting of nucleolin.
Article in Molecular biology of the cell, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 82 citations in OpenAlex.
- Structural Integrity of Nucleolin Is Required to Suppress TDP-43-Mediated Cytotoxicity in Yeast and Human Cell Models.International journal of molecular sciences · 2023Article
- COPI coatomer subunit α-COP interacts with the RNA binding protein Nucleolin via a C-terminal dilysine motif.Human molecular genetics · 2023Article
- Nuclear dynamics: Formation of bodies and trafficking in plant nuclei.Frontiers in plant science · 2022Review
- The glycine arginine-rich domain of the RNA-binding protein nucleolin regulates its subcellular localization.The EMBO journal · 2021Article
- RNA-Binding Proteins in Acute Leukemias.International journal of molecular sciences · 2020Review
- Proteins of the Nucleolus ofCells · 2019Review
- Nucleolin modulates compartmentalization and dynamics of histone 2B-ECFP in the nucleolus.Nucleus (Austin, Tex.) · 2018Article
- Regulation of nucleolus assembly by non-coding RNA polymerase II transcripts.Nucleus (Austin, Tex.) · 2016Review
- A ribonucleoprotein complex protects the interleukin-6 mRNA from degradation by distinct herpesviral endonucleases.PLoS pathogens · 2015Article
- A novel post-translational modification of nucleolin, SUMOylation at Lys-294, mediates arsenite-induced cell death by regulating gadd45α mRNA stability.The Journal of biological chemistry · 2015Article
- Identification and fine mapping of nuclear and nucleolar localization signals within the human ribosomal protein S17.PloS one · 2015Article
- Modulation of hydrogel nanoparticle intracellular trafficking by multivalent surface engineering with tumor targeting peptide.Nanoscale · 2013Article
- Nucleolin protein interacts with microprocessor complex to affect biogenesis of microRNAs 15a and 16.The Journal of biological chemistry · 2011Article
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- Subcellular distribution of human RDM1 protein isoforms and their nucleolar accumulation in response to heat shock and proteotoxic stress.Nucleic acids research · 2007Article
- Insights into nuclear organization in plants as revealed by the dynamic distribution of Arabidopsis SR splicing factors.The Plant cell · 2006Article
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- Novel checkpoint response to genotoxic stress mediated by nucleolin-replication protein a complex formation.Molecular and cellular biology · 2005Article
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Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleolin (713 aa), a major nucleolar protein, presents two structural domains: a N-terminus implicated in interaction with chromatin and a C-terminus containing four RNA-binding domains (RRMs) and a glycine/arginine-rich domain mainly involved in pre-rRNA packaging. Furthermore, nucleolin was shown to shuttle between cytoplasm and nucleolus. To get an insight on the nature of nuclear and nucleolar localization signals, a set of nucleolin deletion mutants in fusion with the prokaryotic chloramphenicol acetyltransferase (CAT) were constructed, and the resulting chimeric proteins were recognized by anti-CAT antibodies. First, a nuclear location signal bipartite and composed of two short basic stretches separated by eleven residues was characterized. Deletion of either motifs renders the protein cytoplasmic. Second, by deleting one or more domains implicated in nucleolin association either with DNA, RNA, or proteins, we demonstrated that nucleolar accumulation requires, in addition to the nuclear localization sequence, at least two of the five RRMs in presence or absence of N-terminus. However, in presence of only one RRM the N-terminus allowed a partial targeting of the chimeric protein to the nucleolus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.