ArticleNucleic acids research1994
Identification of a lens-specific regulatory region (LSR) of the murine alpha B-crystallin gene.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 55 citations in OpenAlex.
- Alpha B-Crystallin in Muscle Disease Prevention: The Role of Physical Activity.Molecules (Basel, Switzerland) · 2022Review
- Article
- Tissue-specific activity of the blind mole rat and the two nucleotide-mutated mouse alphaB-crystallin promoter in transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Potential autoregulation of transcription factor PU.1 by an upstream regulatory element.Molecular and cellular biology · 2005Article
- Sequence and functional conservation of the intergenic region between the head-to-head genes encoding the small heat shock proteins alphaB-crystallin and HspB2 in the mammalian lineage.Journal of molecular evolution · 2004Article
- Functional interactions between alternatively spliced forms of Pax6 in crystallin gene regulation and in haploinsufficiency.Nucleic acids research · 2004Article
- Adaptive evolution of small heat shock protein/alpha B-crystallin promoter activity of the blind subterranean mole rat, Spalax ehrenbergi.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Examination of the molecular basis for the lack of alphaB-crystallin expression in L929 cells.Molecular and cellular biochemistry · 1997Article
- Regulation of the murine alpha B-crystallin/small heat shock protein gene in cardiac muscle.Molecular and cellular biology · 1995Article
- Pax-6 and lens-specific transcription of the chicken delta 1-crystallin gene.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Structure and alternate tissue-preferred transcription initiation of the mouse alpha B-crystallin/small heat shock protein gene.Nucleic acids research · 1994Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous studies have shown that the -661/+44 sequence of the murine alpha B-crystallin gene contains a muscle-preferred enhancer (-426/-257) and can drive the bacterial chloramphenicol acetyltransferase (CAT) gene in the lens, skeletal muscle and heart of transgenic mice. Here we show that transgenic mice carrying a truncated -164/+44 fragment of the alpha B-crystallin gene fused to the CAT gene expressed exclusively in the lens; by contrast mice carrying a -426/+44 fragment of the alpha B gene fused to CAT expressed highly in the lens, skeletal muscle and heart, and slightly in the lung, brain, kidney, spleen and liver. DNase I protection experiments indicated that the -147/-118 sequence is protected by nuclear proteins from alpha TN4-1 lens cell line, but not by nuclear proteins from myotubes of the C2C12 cell line. Site directed mutagenesis of this sequence decreased promoter activity in transiently-transfected lens cells, consistent with this sequence being a lens-specific regulatory region (LSR). We conclude that the -426/-257 enhancer is required for expression in skeletal muscle, heart and possibly other tissues, and that the -164/+44 sequence of the alpha B-crystallin gene is sufficient for expression in the lens of transgenic mice.
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