ArticleMolecular and cellular biology1994
Melanocyte-specific gene expression: role of repression and identification of a melanocyte-specific factor, MSF.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 61 citations in OpenAlex.
- MITF-the first 25 years.Genes & development · 2019Review
- CCN2 modulates hair follicle cycling in mice.Molecular biology of the cell · 2013Article
- TBX2 expression is regulated by PAX3 in the melanocyte lineage.Pigment cell & melanoma research · 2013Article
- A phosphatidylinositol 3-kinase-Pax3 axis regulates Brn-2 expression in melanoma.Molecular and cellular biology · 2012Article
- Mitf dosage as a primary determinant of melanocyte survival after ultraviolet irradiation.Pigment cell & melanoma research · 2009Article
- Pigmentation PAX-ways: the role of Pax3 in melanogenesis, melanocyte stem cell maintenance, and disease.Pigment cell & melanoma research · 2008Review
- Cellular genetic tools to control oncolytic adenoviruses for virotherapy of cancer.Journal of molecular medicine (Berlin, Germany) · 2008Review
- Brn-2 expression controls melanoma proliferation and is directly regulated by beta-catenin.Molecular and cellular biology · 2004Article
- The Brn-2 transcription factor links activated BRAF to melanoma proliferation.Molecular and cellular biology · 2004Article
- The melanocyte inducing factor MITF is stably expressed in cell lines from human clear cell sarcoma.British journal of cancer · 2003Article
- Selective down-regulation of tyrosinase family gene TYRP1 by inhibition of the activity of melanocyte transcription factor, MITF.Nucleic acids research · 2002Article
- Brachyury-related transcription factor Tbx2 and repression of the melanocyte-specific TRP-1 promoter.Molecular and cellular biology · 1998Article
- Regulation of tyrosinase gene expression by cAMP in B16 melanoma cells involves two CATGTG motifs surrounding the TATA box: implication of the microphthalmia gene product.The Journal of cell biology · 1996Article
- Melanocyte-specific expression of the human tyrosinase promoter: activation by the microphthalmia gene product and role of the initiator.Molecular and cellular biology · 1994Article
- Microphthalmia-associated transcription factor as a regulator for melanocyte-specific transcription of the human tyrosinase gene.Molecular and cellular biology · 1994Article
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For a gene to be transcribed in a tissue-specific fashion, expression must be achieved in the appropriate cell type and also be prevented in other tissues. As an approach to understanding the regulation of tissue-specific gene expression, we have analyzed the requirements for melanocyte-specific expression of the tyrosinase-related protein 1 (TRP-1) promoter. Positive regulation of TRP-1 expression is mediated by both an octamer-binding motif and an 11-bp element, termed the M box, which is conserved between the TRP-1 and other melanocyte-specific promoters. We show here that, consistent with its ability to activate transcription in a non-tissue-specific fashion, the M box binds the basic-helix-loop-helix factor USF in vitro. With the use of a combination of site-directed mutagenesis and chimeric promoter constructs, additional elements involved in regulating TRP-1 expression were identified. These include the TATA region, which appears to contribute to the melanocyte specificity of the TRP-1 promoter. Mutational analysis also identified two repressor elements, one at the start site, the other located at -240, which function both in melanoma and nonmelanoma cells. In addition, a melanocyte-specific factor, MSF, binds to sites which overlap both repressor elements, with substitution mutations demonstrating that binding by MSF is not required for repression. Although a functional role for MSF has not been unequivocally determined, the location of its binding sites leads us to speculate that it may act as a melanocyte-specific antirepressor during transcription of the endogenous TRP-1 gene.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.