ArticleMolecular and cellular biology1994
Cloning and characterization of an evolutionarily divergent DNA-binding subunit of mammalian TFIIIC.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 51 citations in OpenAlex.
- Application of quantitative trait locus mapping and transcriptomics to studies of the senescence-accelerated phenotype in rats.BMC genomics · 2014Article
- Contributions of in vitro transcription to the understanding of human RNA polymerase III transcription.Transcription · 2014Review
- Evolution of the B-Block Binding Subunit of TFIIIC That Binds to the Internal Promoter for RNA Polymerase III.International journal of evolutionary biology · 2014Article
- Sirtuin 7 plays a role in ribosome biogenesis and protein synthesis.Molecular & cellular proteomics : MCP · 2014Article
- Different functional modes of p300 in activation of RNA polymerase III transcription from chromatin templates.Molecular and cellular biology · 2008Article
- A test of the model that RNA polymerase III transcription is regulated by selective induction of the 110 kDa subunit of TFIIIC.Nucleic acids research · 2006Article
- Similarities in transcription factor IIIC subunits that bind to the posterior regions of internal promoters for RNA polymerase III.BMC evolutionary biology · 2004Article
- Article
- Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human.Nucleic acids research · 2001Review
- Retinoblastoma protein disrupts interactions required for RNA polymerase III transcription.Molecular and cellular biology · 2000Article
- Identification of a transcription factor IIIA-interacting protein.Nucleic acids research · 2000Article
- Survey and summary: transcription by RNA polymerases I and III.Nucleic acids research · 2000Review
- RNA polymerase III transcription: its control by tumor suppressors and its deregulation by transforming agents.Gene expression · 2000Review
- The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA polymerase III machinery and contains a histone-specific acetyltransferase activity.Molecular and cellular biology · 1999Article
- Cloning and characterization of two evolutionarily conserved subunits (TFIIIC102 and TFIIIC63) of human TFIIIC and their involvement in functional interactions with TFIIIB and RNA polymerase III.Molecular and cellular biology · 1999Article
- Activation of RNA polymerase III transcription in cells transformed by simian virus 40.Molecular and cellular biology · 1999Article
- Effect of vesicular stomatitis virus matrix protein on transcription directed by host RNA polymerases I, II, and III.Journal of virology · 1998Article
- A hydrophobic segment within the 81-amino-acid domain of TFIIIA from Saccharomyces cerevisiae is essential for its transcription factor activity.Molecular and cellular biology · 1998Article
- Tau91, an essential subunit of yeast transcription factor IIIC, cooperates with tau138 in DNA binding.Molecular and cellular biology · 1998Article
- A carboxy-terminal basic region controls RNA polymerase III transcription factor activity of human La protein.Molecular and cellular biology · 1997Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Transcription factor IIIC (TFIIIC) is required for the assembly of a preinitiation complex on 5S RNA, tRNA, and adenovirus VA RNA genes and contains two separable components, TFIIIC1 and TFIIIC2. TFIIIC2 binds to the 3' end of the internal control region of the VAI RNA gene and contains five polypeptides ranging in size from 63 to 220 kDa; the largest of these directly contacts DNA. Here we describe the cloning of cDNAs encoding all (rat) or part (human) of the 220-kDa subunit (TFIIIC alpha). Surprisingly, TFIIIC alpha has no homology to any of the yeast TFIIIC subunits already cloned, suggesting a significant degree of evolutionary divergence for RNA polymerase III factors. Antibodies raised against the N terminus of recombinant human TFIIIC alpha specifically inhibit binding of natural TFIIIC to DNA. Furthermore, immunodepletion assays indicate that TFIIIC alpha is absolutely required for RNA polymerase III transcription of 5S RNA, tRNA, and VAI RNA genes but not for the 7SK RNA and U6 small nuclear RNA genes. Transcription from the tRNA and VAI RNA genes in TFIIIC-depleted nuclear extracts can be restored by addition of purified TFIIIC. In contrast, restoration of 5S RNA gene transcription requires readdition of both TFIIIC and TFIIIA, indicating a promoter-independent interaction between these factors. Immunoprecipitation experiments demonstrate a tight association of all five polypeptides previously identified in the TFIIIC2 fraction, confirming the multisubunit structure of the human factor.
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