ArticleThe Journal of clinical investigation1994
Oscillating activity of a Ca(2+)-sensitive K+ channel. A prerequisite for migration of transformed Madin-Darby canine kidney focus cells.
Article in The Journal of clinical investigation, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 87 citations in OpenAlex.
- Kcnn4/KCa3.1 inhibition blunts polycystic kidney disease progression in mouse models.JCI insight · 2025Article
- Cell Settling, Migration, and Stochastic Cancer Gene Expression Suggest Potassium Membrane Flux May Initiate pH Reversal.Biomolecules · 2025Article
- Unexpected localization of AQP3 and AQP4 induced by migration of primary cultured IMCD cells.Scientific reports · 2021Article
- Ion Channels in Glioma Malignancy.Reviews of physiology, biochemistry and pharmacology · 2021Review
- Review
- Cell volume regulation in cancer cell migration driven by osmotic water flow.Cancer science · 2019Review
- Water Homeostasis and Cell Volume Maintenance and Regulation.Current topics in membranes · 2018Review
- Complementary roles of KCa3.1 channels and β1-integrin during alveolar epithelial repair.Respiratory research · 2015Article
- Intermediate-conductance calcium-activated potassium channel KCa3.1 and chloride channel modulate chemokine ligand (CCL19/CCL21)-induced migration of dendritic cells.Translational research : the journal of laboratory and clinical medicine · 2015Article
- Ion channels and transporters in tumour cell migration and invasion.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Potassium channels in cell cycle and cell proliferation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Evidence of K+ channel function in epithelial cell migration, proliferation, and repair.American journal of physiology. Cell physiology · 2014Review
- Ion channels in regulation of neuronal regenerative activities.Translational stroke research · 2014Review
- The roles of K(+) channels in cancer.Nature reviews. Cancer · 2014Review
- The role of TMEM16A (ANO1) and TMEM16F (ANO6) in cell migration.Pflugers Archiv : European journal of physiology · 2013Article
- Migrating oligodendrocyte progenitor cells swell prior to soma dislocation.Scientific reports · 2013Article
- Regulation of colon cancer cell migration and invasion by CLIC1-mediated RVD.Molecular and cellular biochemistry · 2012Article
- Enhanced expression of ANO1 in head and neck squamous cell carcinoma causes cell migration and correlates with poor prognosis.PloS one · 2012Article
- Regulation of brain tumor dispersal by NKCC1 through a novel role in focal adhesion regulation.PLoS biology · 2012Article
- Calcium-activated potassium channel KCa3.1 in lung dendritic cell migration.American journal of respiratory cell and molecular biology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Migration plays an important role in the formation of tumor metastases. Nonetheless, little is known about electrophysiological phenomena accompanying or underlying migration. Previously, we had shown that in migrating alkali-transformed Madin-Darby canine kidney focus (MDCK-F) cells a Ca(2+)-sensitive 53-pS K+ channel underlies oscillations of the cell membrane potential. The present study defines the role this channel plays in migration of MDCK-F cells. We monitored migration of individual MDCK-F cells by video imaging techniques. Under control conditions, MDCK-F cells migrated at a rate of 0.90 +/- 0.03 microns/min (n = 201). Application of K+ channel blockers (1 and 5 mmol/liter Ba2+, 5 mmol/liter tetraethylammonium, 100 mumol/liter 4-aminopyridine, 5 nmol/liter charybdotoxin) caused marked inhibition of migration, pointing to the importance of K+ channels in migration. Using patch-clamp techniques, we demonstrated the sensitivity of the Ca(2+)-sensitive 53-pS K+ channel to these blockers. Blockade of this K+ channel and inhibition of migration were closely correlated, indicating the necessity of oscillating K+ channel activity for migration. Migration of MDCK-F cells was also inhibited by furosemide or bumetanide, blockers of the Na+/K+/2Cl- cotransporter. We present a model for migration in which oscillations of cell volume play a central role. Whenever they are impaired, migration is inhibited.
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