ArticleThe Journal of cell biology1994
Drosophila singed, a fascin homolog, is required for actin bundle formation during oogenesis and bristle extension.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
110 citing papers in PubMed, 263 citations in OpenAlex.
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- Synergistic assembly, disassembly, and protection of complex forms of bundled F-actin.The Journal of cell biology · 2026Article
- Bitesize bundles F-actin and influences actin remodeling in syncytial Drosophila embryo development.The Journal of cell biology · 2026Article
- Spatially distinct FRL and Ena dependent actin networks coordinate nuclear positioning in Drosophila nurse cells.PLoS genetics · 2026Article
- ATGL-mediated lipid droplet lipolysis promotes collective migration in Drosophila.bioRxiv : the preprint server for biology · 2026Article
- A critical role for the fascin family of actin bundling proteins in axon development, brain wiring and function.Molecular and cellular neurosciences · 2025Article
- The tumor suppressor RASSF8: A WAVE interaction partner controlling migration and cohesion of invasive border cells inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Basal actomyosin pulses expand epithelium coordinating cell flattening and tissue elongation.Nature communications · 2024Article
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- Specific prostaglandins are produced in the migratory cells and the surrounding substrate to promotebioRxiv : the preprint server for biology · 2023Article
- Dusky-like Is Critical for Morphogenesis of the Cellular Protuberances and Formation of the Cuticle inBiology · 2023Article
- Actin Bundles Dynamics and Architecture.Biomolecules · 2023Review
- Specific prostaglandins are produced in the migratory cells and the surrounding substrate to promoteFrontiers in cell and developmental biology · 2023Article
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- A Diaphanous and Enabled-dependent asymmetric actin cable array repositions nuclei during Drosophila oogenesis.Development (Cambridge, England) · 2022Article
- Scale-type-specific requirement for the mosquito Aedes aegypti Spindle-F homologue by regulating microtubule organization.Insect molecular biology · 2022Article
- TheFrontiers in cellular and infection microbiology · 2022Article
- Fascin limits Myosin activity withineLife · 2021Article
- Article
50 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Drosophila singed mutants were named for their gnarled bristle phenotype but severe alleles are also female sterile. Recently, singed protein was shown to have 35% peptide identity with echinoderm fascin. Fascin is found in actin filament bundles in microvilli of sea urchin eggs and in filopodial extensions in coelomocytes. We show that Drosophila singed is required for actin filament bundle formation in the cytoplasm of nurse cells during oogenesis; in severe mutants, the absence of cytoplasmic actin filament bundles allows nurse cell nuclei to lodge in ring canals and block nurse cell cytoplasm transport. Singed is also required for organized actin filament bundle formation in the cellular extension that forms a bristle; in severe mutants, the small disorganized actin filament bundles lack structural integrity and allow bristles to bend and branch during extension. Singed protein is also expressed in migratory cells of the developing egg chamber and in the socket cell of the developing bristle, but no defect is observed in these cells in singed mutants. Purified, bacterially expressed singed protein bundles actin filaments in vitro with the same stoichiometry reported for purified sea urchin fascin. Singed-saturated actin bundles have a molar ratio of singed/actin of approximately 1:4.3 and a transverse cross-banding pattern of 12 nm seen using electron microscopy. Our results suggest that singed protein is required for actin filament bundle formation and is a Drosophila homolog of echinoderm fascin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.