ArticleThe Journal of cell biology1994
COP-coated vesicles are involved in the mitotic fragmentation of Golgi stacks in a cell-free system.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Mitotic Cdc42 waves encode PI(3,4)PScience advances · 2026Article
- Article
- HPV is a cargo for the COPI sorting complex during virus entry.Science advances · 2023Article
- Quantitative Proteomics Analysis of Purified Rat Liver Golgi.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Membrane Curvature, Trans-Membrane Area Asymmetry, Budding, Fission and Organelle Geometry.International journal of molecular sciences · 2020Review
- DjA1 maintains Golgi integrity via interaction with GRASP65.Molecular biology of the cell · 2019Article
- Activators and Effectors of the Small G Protein Arf1 in Regulation of Golgi Dynamics During the Cell Division Cycle.Frontiers in cell and developmental biology · 2018Review
- Golgi structure formation, function, and post-translational modifications in mammalian cells.F1000Research · 2017Review
- Mena-GRASP65 interaction couples actin polymerization to Golgi ribbon linking.Molecular biology of the cell · 2016Article
- Trans-membrane area asymmetry controls the shape of cellular organelles.International journal of molecular sciences · 2015Article
- MEK1 inactivates Myt1 to regulate Golgi membrane fragmentation and mitotic entry in mammalian cells.The EMBO journal · 2013Article
- Signaling at the Golgi during mitosis.Methods in cell biology · 2013Review
- Cell-free reconstitution of vacuole membrane fragmentation reveals regulation of vacuole size and number by TORC1.Molecular biology of the cell · 2012Article
- Quantitative analysis of liver Golgi proteome in the cell cycle.Methods in molecular biology (Clifton, N.J.) · 2012Article
- COPI budding within the Golgi stack.Cold Spring Harbor perspectives in biology · 2011Review
- The ubiquitin ligase HACE1 regulates Golgi membrane dynamics during the cell cycle.Nature communications · 2011Article
- Golgi biogenesis.Cold Spring Harbor perspectives in biology · 2011Review
- Reconstitution of the cell cycle-regulated Golgi disassembly and reassembly in a cell-free system.Nature protocols · 2010Article
- Quantitative proteomics analysis of cell cycle-regulated Golgi disassembly and reassembly.The Journal of biological chemistry · 2010Article
- Molecular mechanism of mitotic Golgi disassembly and reassembly revealed by a defined reconstitution assay.The Journal of biological chemistry · 2008Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rat liver Golgi stacks fragmented when incubated with mitotic but not interphase cytosol in a process dependent on time, temperature, energy (added in the form of ATP) and cdc2 kinase. The cross-sectional length of Golgi stacks fell in the presence of mitotic cytosol by approximately 50% over 30 min without a corresponding decrease in the number of cisternae in the stack. The loss of membrane from stacked and single cisternae occurred with a half-time of approximately 20 min, and was matched by the appearance of both small (50-100 nm in diameter) and large (100-200 nm in diameter) vesicular profiles. Small vesicular profiles constituted more than 50% of the total membrane after 60 min of incubation and they were shown to be vesicles or very short tubules by serial sectioning. In the presence of GTP gamma S all of the small vesicles were COP-coated and both the extent and the rate at which they formed were sufficient to account for the production of small vesicles during mitotic incubation. The involvement of the COP-mediated budding mechanism was confirmed by immunodepletion of one of the subunits of COP coats (the coatomer) from mitotic cytosol. Vesicles were no longer formed but highly fenestrated networks appeared, an effect reversed by the readdition of purified coatomer. Together these experiments provide strong support for our hypothesis that the observed vesiculation of the Golgi apparatus during mitosis in animal cells is caused by continued budding of COP-coated transport vesicles but an inhibition of their fusion with their target membranes.
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