ArticleApplied and environmental microbiology1994
Expression of the lysostaphin gene of Staphylococcus simulans in a eukaryotic system.
Article in Applied and environmental microbiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 31 citations in OpenAlex.
- Antimicrobial peptidase lysostaphin at subinhibitory concentrations modulates staphylococcal adherence, biofilm formation, and toxin production.BMC microbiology · 2023Article
- Self-cleaved expression of recombinant lysostaphin from its cellulose binding domain fusion.Applied microbiology and biotechnology · 2022Article
- Production of Lysostaphin by Nonproprietary Method Utilizing a Promoter from Toxin-Antitoxin System.Molecular biotechnology · 2019Article
- Cloning, Expression, and Purification of Recombinant Lysostaphin From Staphylococcus simulans.Jundishapur journal of microbiology · 2014Article
- Gene and protein sequence optimization for high-level production of fully active and aglycosylated lysostaphin in Pichia pastoris.Applied and environmental microbiology · 2014Article
- Generation of mastitis resistance in cows by targeting human lysozyme gene to β-casein locus using zinc-finger nucleases.Proceedings. Biological sciences · 2014Article
- Lysostaphin: A Staphylococcal Bacteriolysin with Potential Clinical Applications.Pharmaceuticals (Basel, Switzerland) · 2010Review
- Expression of lysostaphin in HeLa cells protects from host cell killing by intracellular Staphylococcus aureus.Medical microbiology and immunology · 2006Article
- The lysostaphin endopeptidase resistance gene (epr) specifies modification of peptidoglycan cross bridges in Staphylococcus simulans and Staphylococcus aureus.Applied and environmental microbiology · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The lysostaphin gene of Staphylococcus simulans was cloned into Escherichia coli. The 5' end of the gene was modified to include a eukaryotic start codon, the Kozak expression start site consensus sequence, and an enzyme site to facilitate manipulation of the gene. Transcription of the modified gene in vitro yielded an RNA transcript which, when added to a rabbit reticulocyte cell-free translation system, directed the synthesis of several products. The largest product, migrating at approximately 93 kDa, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was probably preprolysostaphin, since it was cleaved in the presence of an S. simulans culture supernatant to yield a polypeptide of a size similar to that of mature lysostaphin. When canine pancreatic microsomal vesicles were added to the translation system, translocation of the newly synthesized polypeptides occurred, as judged by protection from proteolysis. The gene was also expressed transiently from the human cytomegalovirus promoter in COS-7 cells. Active enzyme could be detected in the cell lysate, and the prokaryotic signal appeared to target secretion of active enzyme to the culture medium. The successful expression of the lysostaphin gene and processing of the precursor to produce active secreted enzyme open up the possibility of controlling staphylococcal mastitis by targeting expression of this gene to the mammary glands of transgenic animals.
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