ArticleProceedings of the National Academy of Sciences of the United States of America1994
Nuclear import of Agrobacterium VirD2 and VirE2 proteins in maize and tobacco.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 109 citations in OpenAlex.
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- Visualization of VirE2 protein translocation by the Agrobacterium type IV secretion system into host cells.MicrobiologyOpen · 2014Article
- Direct visualization of Agrobacterium-delivered VirE2 in recipient cells.The Plant journal : for cell and molecular biology · 2014Article
- Exploitation of eukaryotic subcellular targeting mechanisms by bacterial effectors.Nature reviews. Microbiology · 2013Review
- Disassembly of synthetic Agrobacterium T-DNA-protein complexes via the host SCF(VBF) ubiquitin-ligase complex pathway.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Agrobacterium may delay plant nonhomologous end-joining DNA repair via XRCC4 to favor T-DNA integration.The Plant cell · 2012Article
- Traversing the Cell: Agrobacterium T-DNA's Journey to the Host Genome.Frontiers in plant science · 2012Article
- Resistance to crown gall disease in transgenic grapevine rootstocks containing truncated virE2 of Agrobacterium.Transgenic research · 2010Article
- VIP1: linking Agrobacterium-mediated transformation to plant immunity?Plant cell reports · 2010Review
- New insights into an old story: Agrobacterium-induced tumour formation in plants by plant transformation.The EMBO journal · 2010Review
- Viral oncolysis that targets Raf-1 signaling control of nuclear transport.Journal of virology · 2010Article
- Agrobacterium tumefaciens and A. rhizogenes use different proteins to transport bacterial DNA into the plant cell nucleus.Microbial biotechnology · 2009Review
- IMPa-4, an Arabidopsis importin alpha isoform, is preferentially involved in agrobacterium-mediated plant transformation.The Plant cell · 2008Article
- Yeast-plant coupled vector system for identification of nuclear proteins.Plant physiology · 2007Article
- The VirE3 protein of Agrobacterium mimics a host cell function required for plant genetic transformation.The EMBO journal · 2005Article
- Expression of plant protein phosphatase 2C interferes with nuclear import of the Agrobacterium T-complex protein VirD2.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Site-specific integration of Agrobacterium tumefaciens T-DNA via double-stranded intermediates.Plant physiology · 2003Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Previously, we have shown that Agrobacterium-plant cell transferred DNA (T-DNA) transport into the host cell nucleus is likely mediated by two specific bacterial proteins, VirD2 and VirE2. Here, we used these proteins to study molecular pathways of nuclear import. First, the role of VirE2 nuclear localization signals (NLSs) in the T-DNA transport pathway was examined by using tobacco plants transgenic for deletion mutants of VirE2. In these plants, the virulence of wild-type Agrobacterium was reduced possibly by competition for the cellular nuclear import machinery. Second, we analyzed the nuclear localization of VirE2 and VirD2 in the nonhost monocot maize. Part of the known recalcitrance of monocots to transformation by Agrobacterium could be due to a potential selectivity in nuclear import pathways in monocotyledonous and dicotyledonous plants. Nuclear transport of VirD2 and VirE2 in maize leaves and roots was compared to that in tobacco protoplasts and roots. Both proteins accumulated in maize leaf and tobacco protoplast nuclei as well as in nuclei of immature root cells. In contrast, VirD2 and VirE2 expressed in mature roots of maize and tobacco remained cytoplasmic. Point mutations of VirE2 nuclear localization signals, NSE 1 and NSE 2, also revealed that, in maize, the NSE 1 signal was mainly responsible for nuclear import; in contrast, both signals functioned independently in tobacco protoplasts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.