ArticleJournal of bacteriology1994
Nucleotide sequence of the rhamnose biosynthetic operon of Shigella flexneri 2a and role of lipopolysaccharide in virulence.
Article in Journal of bacteriology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 66 citations in OpenAlex.
- CRISPR-based mutagenesis of lipopolysaccharide biosynthesis genes in Leptospira interrogans reveals gene essentiality and confirms the role of an O-antigen polymerase.Scientific reports · 2026Article
- Prophages in the infant gut are pervasively induced and may modulate the functionality of their hosts.NPJ biofilms and microbiomes · 2025Article
- Whole genome analysis ofFrontiers in veterinary science · 2025Article
- Molecular and Cellular Mechanisms of Shigella flexneri Dissemination.Frontiers in cellular and infection microbiology · 2016Review
- Glycosylation of the collagen adhesin EmaA of Aggregatibacter actinomycetemcomitans is dependent upon the lipopolysaccharide biosynthetic pathway.Journal of bacteriology · 2010Article
- Polar localization of the autotransporter family of large bacterial virulence proteins.Journal of bacteriology · 2006Article
- Murein lipoprotein is a critical outer membrane component involved in Salmonella enterica serovar typhimurium systemic infection.Infection and immunity · 2005Article
- Sequence analysis of four Shigella boydii O-antigen loci: implication for Escherichia coli and Shigella relationships.Infection and immunity · 2001Article
- Molecular basis of the intracellular spreading of Shigella.Infection and immunity · 2001Review
- Drug targeting Mycobacterium tuberculosis cell wall synthesis: genetics of dTDP-rhamnose synthetic enzymes and development of a microtiter plate-based screen for inhibitors of conversion of dTDP-glucose to dTDP-rhamnose.Antimicrobial agents and chemotherapy · 2001Article
- Development of primers to O-antigen biosynthesis genes for specific detection of Escherichia coli O157 by PCR.Applied and environmental microbiology · 1999Article
- Pathogenic effects of Opolysaccharide from Shigella flexneri strain.World journal of gastroenterology · 1999Article
- Establishment of unipolar localization of IcsA in Shigella flexneri 2a is not dependent on virulence plasmid determinants.Infection and immunity · 1999Article
- Identification of two Shigella flexneri chromosomal loci involved in intercellular spreading.Infection and immunity · 1998Article
- Neural Wiskott-Aldrich syndrome protein is implicated in the actin-based motility of Shigella flexneri.The EMBO journal · 1998Article
- Identification of a genetic locus essential for serotype b-specific antigen synthesis in Actinobacillus actinomycetemcomitans.Infection and immunity · 1998Article
- A gene (wbbL) from Serratia marcescens N28b (O4) complements the rfb-50 mutation of Escherichia coli K-12 derivatives.Journal of bacteriology · 1997Article
- Identification of a fourth gene involved in dTDP-rhamnose synthesis in Streptococcus mutans.Journal of bacteriology · 1997Article
- Biological function of the dTDP-rhamnose synthesis pathway in Streptococcus mutans.Journal of bacteriology · 1997Article
- Identification and characterization of the dTDP-rhamnose biosynthesis and transfer genes of the lipopolysaccharide-related rfb locus in Leptospira interrogans serovar Copenhageni.Journal of bacteriology · 1997Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
N1308, a chromosomal Tn5 mutant of Shigella flexneri 2a, was described previously as a lipopolysaccharide (LPS) mutant with a short O side chain. N1308 formed foci, but not plaques, in LLC-MK2 cell monolayers and was negative in the Serény test. In this study, the wild-type locus inactivated in N1308 was cloned and further defined by means of complementation analysis. A 4.3-kb BstEII-XhoI fragment of S. flexneri 2a YSH6200 DNA was sufficient to restore both normal LPS and virulence phenotype to the mutant. DNA sequencing of this region revealed four genes, rfbA, rfbB, rfbC, and rfbD, encoding the enzymes required for the biosynthesis of activated rhamnose. The four genes were expressed in Escherichia coli, and the expected protein products were visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. N1308 was shown to have normal levels of surface IpaC and IpaD, while a Western blot (immunoblot) of whole-cell lysates or outer membrane fractions indicated an elevated level of appropriately localized VirG. An in vitro invasion assay revealed that N1308 had normal primary invasive capacity and was able to multiply and move normally within the initial infected cell. However, it exhibited a significant reduction in its ability to spread from cell to cell in the monolayer. A double immunofluorescence assay revealed differences between LLC-MK2 cells infected with the wild-type YSH6000 and those infected with N1308. The wild-type bacteria elicited the formation of the characteristic F-actin tails, whereas N1308 failed to do so. However, N1308 was capable of inducing deposition of F-actin, which accumulated in a peribacterial fashion with only slight, if any, unipolar accumulation of the cytoskeletal protein.
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