ArticleThe EMBO journal1994
Role of phospholipase C in Dictyostelium: formation of inositol 1,4,5-trisphosphate and normal development in cells lacking phospholipase C activity.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 103 citations in OpenAlex.
- PIP5K-Ras bistability triggers plasma membrane symmetry breaking to define cellular polarity and regulate migration.bioRxiv : the preprint server for biology · 2025Article
- Inositol phosphate kinases in the eukaryote landscape.Advances in biological regulation · 2021Article
- The origins and evolution of macropinocytosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- Allosteric regulation by cooperative conformational changes of actin filaments drives mutually exclusive binding with cofilin and myosin.Scientific reports · 2016Article
- Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes.Cellular and molecular life sciences : CMLS · 2014Review
- Analysis of Dictyostelium discoideum inositol pyrophosphate metabolism by gel electrophoresis.PloS one · 2014Article
- Phosphoinositides: tiny lipids with giant impact on cell regulation.Physiological reviews · 2013Review
- Delineating the core regulatory elements crucial for directed cell migration by examining folic-acid-mediated responses.Journal of cell science · 2013Article
- The IplA Ca2+ channel of Dictyostelium discoideum is necessary for chemotaxis mediated through Ca2+, but not through cAMP, and has a fundamental role in natural aggregation.Journal of cell science · 2012Article
- The antiepileptic drug valproic acid and other medium-chain fatty acids acutely reduce phosphoinositide levels independently of inositol in Dictyostelium.Disease models & mechanisms · 2012Article
- Prolyl oligopeptidase, inositol phosphate signalling and lithium sensitivity.CNS & neurological disorders drug targets · 2011Review
- Oscillatory signaling and network responses during the development of Dictyostelium discoideum.Ageing research reviews · 2008Review
- Directional sensing during chemotaxis.FEBS letters · 2008Review
- The regulation of cell motility and chemotaxis by phospholipid signaling.Journal of cell science · 2008Review
- Phospholipase C regulation of phosphatidylinositol 3,4,5-trisphosphate-mediated chemotaxis.Molecular biology of the cell · 2007Article
- Essential role of PI3-kinase and phospholipase A2 in Dictyostelium discoideum chemotaxis.The Journal of cell biology · 2007Article
- Chemoattractants and chemorepellents act by inducing opposite polarity in phospholipase C and PI3-kinase signaling.The Journal of cell biology · 2007Article
- Guanylyl cyclase protein and cGMP product independently control front and back of chemotaxing Dictyostelium cells.Molecular biology of the cell · 2006Article
- Release of Ca2+ from the endoplasmic reticulum contributes to Ca2+ signaling in Dictyostelium discoideum.Eukaryotic cell · 2005Article
- A link of Ca2+ to cAMP oscillations in Dictyostelium: the calmodulin antagonist W-7 potentiates cAMP relay and transiently inhibits the acidic Ca2+-store.BMC developmental biology · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The micro-organism Dictyostelium uses extracellular cAMP to induce chemotaxis and cell differentiation. Signals are transduced via surface receptors, which activate G proteins, to effector enzymes. The deduced protein sequence of Dictyostelium discoideum phosphatidylinositol-specific phospholipase C (PLC) shows strong homology with the mammalian PLC-delta isoforms. To study the role of PLC in Dictyostelium, a plc- mutant was constructed by disruption of the PLC gene. No basal or stimulated PLC activity could be measured during the whole developmental programme of the plc- cells. Loss of PLC activity did not result in a visible alteration of growth or development. Further analysis showed that developmental gene regulation, cAMP-mediated chemotaxis and activation of guanylyl and adenylyl cyclase were normal. Although the cells lack PLC activity, inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] was present at only slightly lower concentrations compared with control cells. Mass analysis of inositol phosphates demonstrated the presence of a broad spectrum of inositol phosphates in Dictyostelium, which was unaltered in the plc- mutant. Cell labelling experiments with [3H]inositol indicated that [3H]Ins(1,4,5)P3 was formed in a different manner in the mutant than in control cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.