ArticleCell calcium1994
Fluorescence lifetime imaging of intracellular calcium in COS cells using Quin-2.
Article in Cell calcium, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 107 citations in OpenAlex.
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- A method for estimating intracellular ion concentration using optical nanosensors and ratiometric imaging.Scientific reports · 2017Article
- Effect of Ca²⁺ on the steady-state and time-resolved emission properties of the genetically encoded fluorescent sensor CatchER.The journal of physical chemistry. B · 2015Article
- Temporal Data Set Reduction Based on D-Optimality for Quantitative FLIM-FRET Imaging.PloS one · 2015Article
- Reduced temporal sampling effect on accuracy of time-domain fluorescence lifetime Förster resonance energy transfer.Journal of biomedical optics · 2014Article
- Asante Calcium Green and Asante Calcium Red--novel calcium indicators for two-photon fluorescence lifetime imaging.PloS one · 2014Article
- Nanoparticle PEBBLE sensors for quantitative nanomolar imaging of intracellular free calcium ions.Analytical chemistry · 2012Article
- Effects of incomplete decay in fluorescence lifetime estimation.Biomedical optics express · 2011Article
- Segmented frequency-domain fluorescence lifetime measurements: minimizing the effects of photobleaching within a multi-component system.Journal of fluorescence · 2007Article
- Detectors for single-molecule fluorescence imaging and spectroscopy.Journal of modern optics · 2007Article
- Multi-dimensional time-correlated single photon counting (TCSPC) fluorescence lifetime imaging microscopy (FLIM) to detect FRET in cells.Journal of microscopy · 2004Article
- Fluorescence lifetime-resolved pH imaging of living cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2003Article
- Texture analysis of fluorescence lifetime images of AT- and GC-rich regions in nuclei.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2001Article
- Texture analysis of fluorescence lifetime images of nuclear DNA with effect of fluorescence resonance energy transfer.Cytometry · 2001Article
- Fluorescence lifetime imaging of nuclear DNA: effect of fluorescence resonance energy transfer.Cytometry · 2000Article
- Global analysis of fluorescence lifetime imaging microscopy data.Biophysical journal · 2000Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We describe the first fluorescence lifetime images of cells. To demonstrate this new capability we measured intracellular images of Ca2+ in COS cells based on the Ca(2+)-dependent fluorescence lifetime of Quin-2. Apparent fluorescence lifetimes were measured by the phase-modulation method using a gain-modulated image intensifier and a slow-scan CCD camera. We describe methods to correct the images for photobleaching during acquisition of the data, and to correct for the position-dependent response of the image intensifier. The phase angle Quin-2 images were found to yield lower than expected Ca2+ concentrations, which appears to be the result of the formation of fluorescent photoproducts by Quin-2. Fluorescence lifetime imaging (FLIM) does not require wavelength-radiometric probes and appears to provide new opportunities for chemical imaging of cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.