ArticleProceedings of the National Academy of Sciences of the United States of America1994
Essential role for a heterogeneous nuclear ribonucleoprotein (hnRNP) in oogenesis: hrp40 is absent from the germ line in the dorsoventral mutant squid.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 58 citations in OpenAlex.
- The RNA-binding protein Squid regulates embryonic midgut development via Axin alternative splicing in Bombyx mori.Communications biology · 2026Article
- Alternative splicing of squid is associated with sex-ratio allocation in the wasp Copidosomopsis nacoleiae.BMC genomics · 2025Article
- In silico identification, characterization, and expression analysis of RNA recognition motif (RRM) containing RNA-binding proteins in Aedes aegypti.Parasitology research · 2023Article
- Novel roles for RNA binding proteins squid, hephaesteus, and Hrb27C in Drosophila oogenesis.Developmental dynamics : an official publication of the American Association of Anatomists · 2023Article
- Drosophila suppressor of sable protein [Su(s)] promotes degradation of aberrant and transposon-derived RNAs.Molecular and cellular biology · 2009Article
- The long journey of actin and actin-associated proteins from genes to polysomes.Cellular and molecular life sciences : CMLS · 2009Review
- Squid, Cup, and PABP55B function together to regulate gurken translation in Drosophila.Developmental biology · 2008Article
- Heterogeneous nuclear ribonucleoprotein A/B and G inhibits the transcription of gonadotropin-releasing-hormone 1.Molecular and cellular neurosciences · 2008Article
- Article
- Mouse homologue of the human SART3 gene encoding tumor-rejection antigen.Japanese journal of cancer research : Gann · 2000Article
- Article
- Specific isoforms of squid, a Drosophila hnRNP, perform distinct roles in Gurken localization during oogenesis.Genes & development · 1999Article
- Separable roles in vivo for the two RNA binding domains of Drosophila A1-hnRNP homolog.RNA (New York, N.Y.) · 1998Article
- RNA-binding protein TIAR is essential for primordial germ cell development.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Mutations in the hrp48 gene, which encodes a Drosophila heterogeneous nuclear ribonucleoprotein particle protein, cause lethality and developmental defects and affect P-element third-intron splicing in vivo.Molecular and cellular biology · 1997Article
- Interaction between the human nuclear cap-binding protein complex and hnRNP F.Molecular and cellular biology · 1997Article
- Structure, tissue distribution and genomic organization of the murine RRM-type RNA binding proteins TIA-1 and TIAR.Nucleic acids research · 1996Article
- Altered levels of the Drosophila HRB87F/hrp36 hnRNP protein have limited effects on alternative splicing in vivo.Molecular biology of the cell · 1996Article
- Function of conserved domains of hnRNP A1 and other hnRNP A/B proteins.The EMBO journal · 1994Article
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Drosophila melanogaster hrp40 proteins are abundant nuclear pre-mRNA-binding proteins that are similar to the heterogeneous nuclear ribonucleoprotein (hnRNP) A/B proteins of vertebrates. Recently, hrp40 has been shown to be encoded by the squid gene, which is required for dorsoventral axis formation during oogenesis. Eggs and embryos from homozygous squid mothers are severely dorsalized, and complete deletion of the squid gene results in lethality. Here we have examined the expression and localization of hrp40 in wild-type and squid mutant ovaries. Using a monoclonal antibody specific for hrp40, the same isoforms of hrp40 are detected in both wild-type and squid ovaries, but the amount of hrp40 is reduced in squid ovaries. Furthermore, immunolocalization of hrp40 in wild-type egg chambers shows that hrp40 is present in the nurse cells, oocyte, and follicle cells. In contrast, in squid mutant egg chambers, hrp40 is absent from the germ-line-derived nurse cells and oocyte, but it is detected in the somatic follicle cells. The absence of hrp40 from the germ-line-derived cells of developing egg chambers is likely to lead to the striking dorsalized phenotype of squid eggs. In addition, dramatic stage-specific changes in the cellular localization of hrp40 are seen; the protein found in the nurse cell nuclei during early stages of oogenesis migrates to the cytoplasm at later stages. These findings reveal dynamic patterns of expression and localization of hnRNP proteins during development and provide evidence for an essential role for hnRNP proteins.
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