ArticleProceedings of the National Academy of Sciences of the United States of America1994
Preparation and screening of an arrayed human genomic library generated with the P1 cloning system.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 130 citations in OpenAlex.
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- Validation of DNA probes for molecular cytogenetics by mapping onto immobilized circular DNA.Molecular cytogenetics · 2008Article
- A humanized mouse model for the reduced folate carrier.Molecular genetics and metabolism · 2008Article
- Hypermethylation of the spleen tyrosine kinase promoter in T-lineage acute lymphoblastic leukemia.Oncogene · 2003Article
- Localization of the Fanconi anemia complementation group D gene to a 200-kb region on chromosome 3p25.3.American journal of human genetics · 2000Article
- Rational design of landmark probes for quantitative DNA fiber mapping (QDFM).Nucleic acids research · 2000Article
- A physical map, including a BAC/PAC clone contig, of the Williams-Beuren syndrome--deletion region at 7q11.23.American journal of human genetics · 2000Article
- Human RNA-specific adenosine deaminase ADAR1 transcripts possess alternative exon 1 structures that initiate from different promoters, one constitutively active and the other interferon inducible.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Frequent genetic alterations in simple urothelial hyperplasias of the bladder in patients with papillary urothelial carcinoma.The American journal of pathology · 1999Article
- p73 at chromosome 1p36.3 is lost in advanced stage neuroblastoma but its mutation is infrequent.Oncogene · 1999Article
- Mapping ESTs by fiber-FISH.Genome research · 1999Article
- Transfer of P1 inserts into a yeast-bacteria shuttle vector by co-transformation mediated homologous recombination.Nucleic acids research · 1998Article
- Modification of bacterial artificial chromosome clones using Cre recombinase: introduction of selectable markers for expression in eukaryotic cells.Genome research · 1998Article
- Isolating large nested deletions in bacterial and P1 artificial chromosomes by in vivo P1 packaging of products of Cre-catalysed recombination between the endogenous and a transposed loxP site.Nucleic acids research · 1997Article
- A nucleotide substitution in the promoter of human angiotensinogen is associated with essential hypertension and affects basal transcription in vitro.The Journal of clinical investigation · 1997Article
- Angelman syndrome associated with an inversion of chromosome 15q11.2q24.3.American journal of human genetics · 1997Article
- Cloning of the human NADH: ubiquinone oxidoreductase subunit B13: localization to chromosome 7q32 and identification of a pseudogene on 11p15.Mammalian genome : official journal of the International Mammalian Genome Society · 1997Article
- Cloning of the cDNA for a human homologue of the Drosophila white gene and mapping to chromosome 21q22.3.American journal of human genetics · 1996Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe here the construction and initial characterization of a 3-fold coverage genomic library of the human haploid genome that was prepared using the bacteriophage P1 cloning system. The cloned DNA inserts were produced by size fractionation of a Sau3AI partial digest of high molecular weight genomic DNA isolated from primary cells of human foreskin fibroblasts. The inserts were cloned into the pAd10sacBII vector and packaged in vitro into P1 phage. These were used to generate recombinant bacterial clones, each of which was picked robotically from an agar plate into a well of a 96-well microtiter dish, grown overnight, and stored at -70 degrees C. The resulting library, designated DMPC-HFF#1 series A, consists of approximately 130,000-140,000 recombinant clones that were stored in 1500 microtiter dishes. To screen the library, clones were combined in a pooling strategy and specific loci were identified by PCR analysis. On average, the library contains two or three different clones for each locus screened. To date we have identified a total of 17 clones containing the hypoxanthine-guanine phosphoribosyltransferase, human serum albumin-human alpha-fetoprotein, p53, cyclooxygenase I, human apurinic endonuclease, beta-polymerase, and DNA ligase I genes. The cloned inserts average 80 kb in size and range from 70 to 95 kb, with one 49-kb insert and one 62-kb insert.
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