ArticleProceedings of the National Academy of Sciences of the United States of America1994
Formation of 4-hydroxy-2-nonenal-modified proteins in the renal proximal tubules of rats treated with a renal carcinogen, ferric nitrilotriacetate.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers, 1 of them a synthesis that pooled it.
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Who cites it
68 citing papers in PubMed, 1 synthesis or guideline pooled it, 334 citations in OpenAlex.
- A systematic review of molecular approaches that link mitochondrial dysfunction and neuroinflammation in Parkinson's disease.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2021Pooled it
- Ferroptosis and cancer: when iron turns against tumors.Cellular and molecular life sciences : CMLS · 2025Review
- Urinary liver-type fatty acid-binding protein level as a prognostic indicator of acute kidney injury secondary to severe falciparum malaria.CEN case reports · 2024Article
- Molecular mechanism of formation and destruction of a pseudo‑capsule in clear cell renal cell carcinoma.Oncology letters · 2024Article
- Environmental impact on carcinogenesis under BRCA1 haploinsufficiency.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2023Review
- Iron as spirit of life to share under monopoly.Journal of clinical biochemistry and nutrition · 2022Article
- BRCA1 haploinsufficiency promotes chromosomal amplification under Fenton reaction-based carcinogenesis through ferroptosis-resistance.Redox biology · 2022Article
- Interaction between macrophages and ferroptosis.Cell death & disease · 2022Review
- Carcinogenesis as Side Effects of Iron and Oxygen Utilization: From the Unveiled Truth toward Ultimate Bioengineering.Cancers · 2020Review
- Asbestos conceives Fe(II)-dependent mutagenic stromal milieu through ceaseless macrophage ferroptosis and β-catenin induction in mesothelium.Redox biology · 2020Article
- Quantitation of Apurinic/Apyrimidinic Sites in Isolated DNA and in Mammalian Tissue with a Reduced Level of Artifacts.Analytical chemistry · 2019Article
- Iron catalysis of lipid peroxidation in ferroptosis: Regulated enzymatic or random free radical reaction?Free radical biology & medicine · 2019Review
- Endometriotic cyst fluid induces reactive oxygen species (ROS) in human immortalized epithelial cells derived from ovarian endometrioma.Redox report : communications in free radical research · 2017Article
- The potential protective effect of Commelina nudiflora L. against carbon tetrachloride (CClEnvironmental health and preventive medicine · 2017Article
- Activation of proinflammatory signaling by 4-hydroxynonenal-Src adducts in aged kidneys.Oncotarget · 2016Article
- Role of hemoglobin and transferrin in multi-wall carbon nanotube-induced mesothelial injury and carcinogenesis.Cancer science · 2016Article
- Pain-reducing anesthesia prevents oxidative stress in human term placenta.Journal of clinical biochemistry and nutrition · 2016Article
- Ovarian endometriosis-associated stromal cells reveal persistently high affinity for iron.Redox biology · 2015Article
- MAPKs' status at early stages of renal carcinogenesis and tumors induced by ferric nitrilotriacetate.Molecular and cellular biochemistry · 2015Article
- 4-Hydroxynonenal in the pathogenesis and progression of human diseases.Current medicinal chemistry · 2014Review
8 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An iron chelate, ferric nitrilotriacetate (Fe-NTA), induces proximal tubular necrosis, a consequence of lipid peroxidation, that finally leads to a high incidence of renal adenocarcinoma in rodents. Lipid peroxidation as monitored by formation of thiobarbituric acid-reactive substances and free 4-hydroxy-2-nonenal (HNE) was observed in the kidney homogenates of rats treated with Fe-NTA. Based on the fact that HNE is capable of reacting with cellular proteins, we attempted to detect the localization of HNE-modified proteins in rat kidney tissues with an immunohistochemical procedure. By means of an immunohistochemical technique using polyclonal antibody against the HNE-modified proteins, it was shown that HNE-modified proteins are formed in the target cells of this carcinogenesis model. HNE-modified proteins were detected in the renal proximal tubules 1 hr after i.p. administration of Fe-NTA (15 mg of iron per kg). Intense positivity was found in the cells with degeneration. After 6 hr, the level of HNE-protein conjugates decreased due to the subsequent necrosis. The intensity of the immunochemical reaction with HNE-modified proteins increased in parallel with an increase in the amounts of thiobartituric acid-reactive substances and free HNE that were found. Furthermore, histochemical detection of aldehydes by cold Schiff's reagent demonstrated that location of aldehydes was identical to that of the HNE-modified proteins determined by immunohistochemical procedures. It would thus appear that the production of HNE, a genotoxic and mutagenic aldehyde, and its reaction with proteins may play a role in Fe-NTA-induced renal carcinogenesis.
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