ArticleProceedings of the National Academy of Sciences of the United States of America1994
Direct selection for a catalytic mechanism from combinatorial antibody libraries.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 87 citations in OpenAlex.
- Advancing the immunoaffinity platform AFFIRM to targeted measurements of proteins in serum in the pg/ml range.PloS one · 2018Article
- Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Induced-fit catalysis of corannulene bowl-to-bowl inversion.Nature chemistry · 2014Article
- Routes to covalent catalysis by reactive selection for nascent protein nucleophiles.Journal of the American Chemical Society · 2007Article
- Selecting and screening recombinant antibody libraries.Nature biotechnology · 2005Review
- Molecular evolution in static and dynamical landscapes.Journal of biological physics · 2002Article
- Generation of recombinant antibodies.Molecular biotechnology · 1999Review
- Selection for improved subtiligases by phage display.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- A method for directed evolution and functional cloning of enzymes.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Making chemistry selectable by linking it to infectivity.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- In vivo versus in vitro screening or selection for catalytic activity in enzymes and abzymes.Molecular biotechnology · 1997Review
- Selection of proteins and peptides from libraries displayed on filamentous bacteriophage.Molecular biotechnology · 1996Review
- Synthetic human antibodies.Nature medicine · 1995Review
- Complement-specific antibodies: designing novel anti-inflammatories.Nature medicine · 1995Review
- Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Semisynthetic combinatorial antibody library methodology in the phage-display format was used to select for a cysteine residue in complementarity-determining regions. Libraries were panned with an alpha-phenethyl pyridyl disulfide that undergoes disulfide interchange. Out of 10 randomly picked clones, two contained an unpaired cysteine, one of which was studied. The antibody catalyzed the hydrolysis of the corresponding thioester where the electrophilic carbonyl occupies the three-dimensional space that was defined by the reactive sulfur atom during selection. The reaction operates by covalent catalysis. Although the steady-state rate enhancement relative to the activated thiol ester substrate is modest, hydrolysis of the acylated cysteine intermediate is remarkably efficient with a catalytic advantage of about four orders of magnitude. The results suggest that iterative mechanism-based selection procedures can recapitulate the enzymatic mechanisms refined through evolution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.