ArticleProceedings of the National Academy of Sciences of the United States of America1994
The zinc finger region of the adenovirus E1A transactivating domain complexes with the TATA box binding protein.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed.
- Article
- Article
- The Transcriptional Repressor BS69 is a Conserved Target of the E1A Proteins from Several Human Adenovirus Species.Viruses · 2018Article
- Review
- Adenovirus E1A recruits the human Paf1 complex to enhance transcriptional elongation.Journal of virology · 2014Article
- Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex.Journal of virology · 2013Article
- Viral retasking of hBre1/RNF20 to recruit hPaf1 for transcriptional activation.PLoS pathogens · 2013Article
- Distribution and dynamics of transcription-associated proteins during parvovirus infection.Journal of virology · 2012Article
- Cellular GCN5 is a novel regulator of human adenovirus E1A-conserved region 3 transactivation.Journal of virology · 2012Article
- Comparison of E1A CR3-dependent transcriptional activation across six different human adenovirus subgroups.Journal of virology · 2010Article
- Unique sequence features of the Human adenovirus 31 complete genomic sequence are conserved in clinical isolates.BMC genomics · 2009Article
- Transcriptional control by adenovirus E1A conserved region 3 via p300/CBP.Nucleic acids research · 2009Article
- Article
- Transactivation, dimerization, and DNA-binding activity of white spot syndrome virus immediate-early protein IE1.Journal of virology · 2008Article
- Identification of a second CtBP binding site in adenovirus type 5 E1A conserved region 3.Journal of virology · 2008Article
- Intrinsic structural disorder in adenovirus E1A: a viral molecular hub linking multiple diverse processes.Journal of virology · 2008Review
- Efficient and reproducible generation of high-expressing, stable human cell lines without need for antibiotic selection.BMC biotechnology · 2008Article
- The landscape of human proteins interacting with viruses and other pathogens.PLoS pathogens · 2008Article
- Article
- Roles for APIS and the 20S proteasome in adenovirus E1A-dependent transcription.The EMBO journal · 2006Article
6 more citing papers are in PubMed but not listed here.
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4 authors.
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Abstract
The 289R E1A protein of adenovirus transactivates a variety of viral and cellular promoters through protein-protein interactions. In earlier studies, mutational analyses of the E1A transactivating domain identified residues that are critical for transactivation and implied that the zinc finger region of the transactivating domain binds a transcription factor. Also, the E1A activation domain was found to bind to the TATA box binding protein (TBP) in vitro. Here, we tested the significance of the E1A-TBP interaction for E1A transactivation by analyzing the effects of conservative substitutions at each of the 49 residues of the E1A activation domain. Seven of the substitutions significantly diminished TBP binding in vitro. All of these were in the zinc finger region and were defective for transactivation in vivo. The perfect correlation between reduced TBP binding and transactivation argues strongly that a direct interaction between the E1A activation domain and TBP is critical to the mechanism of E1A activation. This genetic analysis leads us to further suggest that another factor, which is limiting, is also necessary for E1A-mediated transactivation.
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