ArticleThe Biochemical journal1994
Platelet-derived growth factor-induced phosphatidylinositol 3-kinase activation mediates actin rearrangements in fibroblasts.
Article in The Biochemical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 133 citations in OpenAlex.
- Roles for 3' Phosphoinositides in Macropinocytosis.Sub-cellular biochemistry · 2022Article
- The Crossroads between RAS and RHO Signaling Pathways in Cellular Transformation, Motility and Contraction.Genes · 2021Review
- Shear Stress Accumulation Enhances von Willebrand Factor-Induced Platelet P-Selectin Translocation in a PI3K/Akt Pathway-Dependent Manner.Frontiers in cell and developmental biology · 2021Article
- Protein kinases: master regulators of neuritogenesis and therapeutic targets for axon regeneration.Cellular and molecular life sciences : CMLS · 2020Review
- Dorsal ruffles enhance activation of Akt by growth factors.Journal of cell science · 2018Article
- A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.Nature communications · 2018Article
- Regulation of circular dorsal ruffles, macropinocytosis, and cell migration by RhoG and its exchange factor, Trio.Molecular biology of the cell · 2017Article
- The chemistry and biology of mycolactones.Beilstein journal of organic chemistry · 2017Review
- Crossroads of PI3K and Rac pathways.Small GTPases · 2015Review
- Mechanistic insights into the regulation of circular dorsal ruffle formation.Journal of biochemistry · 2013Review
- Functions and regulation of circular dorsal ruffles.Molecular and cellular biology · 2012Review
- PI3K signalling: the path to discovery and understanding.Nature reviews. Molecular cell biology · 2012Article
- Protein kinase A regulates 3-phosphatidylinositide dynamics during platelet-derived growth factor-induced membrane ruffling and chemotaxis.The Journal of biological chemistry · 2008Article
- PTEN tumor suppressor associates with NHERF proteins to attenuate PDGF receptor signaling.The EMBO journal · 2006Article
- Quantitative elucidation of a distinct spatial gradient-sensing mechanism in fibroblasts.The Journal of cell biology · 2005Article
- Spatial analysis of 3' phosphoinositide signaling in living fibroblasts: II. Parameter estimates for individual cells from experiments.Biophysical journal · 2004Article
- Phosphatidylinositol 3-kinase is required for the expression but not for the induction or the maintenance of long-term potentiation in the hippocampal CA1 region.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Tumor necrosis factor-alpha induces stress fiber formation through ceramide production: role of sphingosine kinase.Molecular biology of the cell · 2001Article
- A regulatory role for Src homology 2 domain-containing inositol 5'-phosphatase (SHIP) in phagocytosis mediated by Fc gamma receptors and complement receptor 3 (alpha(M)beta(2); CD11b/CD18).The Journal of experimental medicine · 2001Article
- Corequirement of specific phosphoinositides and small GTP-binding protein Cdc42 in inducing actin assembly in Xenopus egg extracts.The Journal of cell biology · 1998Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Various agonist-induced cell responses in neutrophils and fibroblasts, such as chemotaxis and cytoskeletal rearrangements, have been shown to correlate with the synthesis of PtdIns(3,4,5)P3; however, the significance of this rise in second messenger levels is not clear. We show here that wortmannin inhibits platelet-derived growth factor (PDGF)-mediated production of PtdIns(3,4,5)P3 in human foreskin fibroblasts with an IC50 of about 5 nM. A similar inhibition was observed in in vitro assays (IC50 approximately 1 nM) with phosphatidylinositol 3-kinase immunoprecipitated by antibodies directed against the 85 kDa subunit (p85). On the other hand, wortmannin did not affect PDGF-mediated phosphorylation of p85 as detected by immunoprecipitation with anti-phosphotyrosine antibodies, and did not dissociate the complex of p85 and the catalytic subunit (p110) of phosphatidylinositol 3-kinase. These results are consistent with a direct, specific inhibition of the enzyme by wortmannin at concentrations relevant for its previously reported effects on cellular responses. When stimulated with PDGF, human foreskin fibroblasts form circular structures of filamentous actin. Preincubation of these cells with wortmannin inhibits PDGF-mediated actin rearrangements, suggesting a need for PtdIns(3,4,5)P3 formation as a signal for this cell response.
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