ArticleNucleic acids research1994
The 5' flanking region of the rat LAP (C/EBP beta) gene can direct high-level, position-independent, copy number-dependent expression in multiple tissues in transgenic mice.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 55 citations in OpenAlex.
- Animal models of maple syrup urine disease.Journal of inherited metabolic disease · 2009Review
- Liver-specific expression of interferon gamma following adenoviral gene transfer controls hepatitis B virus replication in mice.Gene therapy · 2005Article
- Stringent doxycycline dependent control of CRE recombinase in vivo.Nucleic acids research · 2002Article
- Locus control regions.Blood · 2002Review
- Dividing the empire: boundary chromatin elements delimit the territory of enhancers.The EMBO journal · 1999Review
- A potential role for the nuclear factor of activated T cells family of transcriptional regulatory proteins in adipogenesis.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Human matrix attachment regions insulate transgene expression from chromosomal position effects in Drosophila melanogaster.Molecular and cellular biology · 1998Article
- The DBP gene is expressed according to a circadian rhythm in the suprachiasmatic nucleus and influences circadian behavior.The EMBO journal · 1997Article
- Analysis of the chromatin domain organisation around the plastocyanin gene reveals an MAR-specific sequence element in Arabidopsis thaliana.Nucleic acids research · 1997Article
- A locus control region at -12 kb of the tyrosinase gene.The EMBO journal · 1996Article
- Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Targeted transgenesis.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Contrasting effects of alpha and beta globin regulatory elements on chromatin structure may be related to their different chromosomal environments.The EMBO journal · 1995Article
- Targeting gene expression to haemopoietic stem cells: a chromatin-dependent upstream element mediates cell type-specific expression of the stem cell antigen CD34.The EMBO journal · 1995Article
- Analysis of a 70 kb segment of DNA containing the human zeta and alpha-globin genes linked to their regulatory element (HS-40) in transgenic mice.Nucleic acids research · 1994Article
- The effect of matrix attached regions (MAR) and specialized chromatin structure (SCS) on the expression of gene constructs in cultured cells and in transgenic mice.Molecular biology reportsArticle
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3 authors at 1 institution in 1 country.
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Abstract
The efficiency and tissue-specificity of transgene expression in animals is usually subject to the position of integration into the host chromatin. We have discovered that a 2.8kbp fragment flanking the rat gene encoding the transcription factor LAP (C/EBP beta) directs position-independent, copy number-dependent expression in transgenic-mouse livers. Concomitantly, the DNAse I hypersensitivity pattern normally observed in the liver is established in the integrated transgene construct demonstrating that this region is capable of creating chromatin structures equivalent to the endogenous situation. These observations are reminiscent of the locus control regions (LCR) described for several genes. Additionally, this LAP element functions with both intron-less and intron-containing genes. The tissue specificity of this element, however, is not restricted to liver. The 2.8kbp region is capable of allowing position-independent, copy number-dependent expression in brain, kidney, heart, spleen, and lung, but in a construct-dependent manner. This is, to our knowledge, the first transcription factor gene with which a cis-linked LCR-like element has been associated.
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