ArticleMolecular and cellular biology1994
Sequences containing the second-intron enhancer are essential for transcription of the human apolipoprotein B gene in the livers of transgenic mice.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 53 citations in OpenAlex.
- The bread wheat epigenomic map reveals distinct chromatin architectural and evolutionary features of functional genetic elements.Genome biology · 2019Article
- The dynamic architectural and epigenetic nuclear landscape: developing the genomic almanac of biology and disease.Journal of cellular physiology · 2014Review
- Molecular analysis of the first intron in the bovine myostatin gene.Molecular biology reports · 2011Article
- Transgene design.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Unique genes in plants: specificities and conserved features throughout evolution.BMC evolutionary biology · 2008Article
- Transcriptional regulation of lipopolysaccharide (LPS)-induced Toll-like receptor (TLR) expression in murine macrophages: role of interferon regulatory factors 1 (IRF-1) and 2 (IRF-2).Journal of endotoxin research · 2006Article
- The human vitamin D-binding protein gene contains locus control determinants sufficient for autonomous activation in hepatic chromatin.Nucleic acids research · 2006Article
- Inappropriate splicing of a chimeric gene containing a large internal exon results in exon skipping in transgenic mice.Nucleic acids research · 1996Article
- Contrasting effects of the SATB1 core nuclear matrix attachment region and flanking sequences of the keratin 18 gene in transgenic mice.Molecular biology of the cell · 1996Article
- Position-independent transgene expression mediated by boundary elements from the apolipoprotein B chromatin domain.Molecular and cellular biology · 1995Article
- The effect of matrix attached regions (MAR) and specialized chromatin structure (SCS) on the expression of gene constructs in cultured cells and in transgenic mice.Molecular biology reportsArticle
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
To identify DNA sequence elements from the human apolipoprotein B (apoB) gene required for high-level, correct tissue-specific expression in transgenic mice, we made several constructs that included one or more of the key regulatory elements that were previously characterized with cultured liver-derived and intestine-derived cell lines. Our data show that the apoB promoter alone (-898 to +121) is not sufficient to direct transcription in transgenic mice. An enhancer located in the second intron is absolutely required to specify transcription by the homologous apoB promoter in the livers of transgenic mice; this enhancer does not direct transcription in the small intestines. Thus, the elements controlling transcriptional activation of the apoB gene in the liver and the intestine in vivo are distinct and separable. Analysis of the DNase I hypersensitivity of the integrated human transgenes in various lines of expressing and nonexpressing mice suggests that the formation of DH4, a strong hypersensitive site in intron 2, may be a prerequisite for hepatic expression of the apoB gene. Nuclear matrix association regions (MARs) of the apoB gene may play a role in transgene expression. Constructs including MAR sequences displayed higher levels of expression than those lacking them. However, these MARs did not completely insulate the associated transgenes from position effects.
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