ArticleJournal of pharmacokinetics and biopharmaceutics1993
Physiological pharmacokinetics of solutes in the isolated perfused rat hindlimb: characterization of the physiology with changing perfusate flow, protein content, and temperature using statistical moment analysis.
Article in Journal of pharmacokinetics and biopharmaceutics, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 34 citations in OpenAlex.
- Hepatocellular necrosis, fibrosis and microsomal activity determine the hepatic pharmacokinetics of basic drugs in right-heart-failure-induced liver damage.Pharmaceutical research · 2012Article
- Drug structure-transport relationships.Journal of pharmacokinetics and pharmacodynamics · 2010Review
- Characterization of the physiological spaces and distribution of tolbutamide in the perfused rat pancreas.Pharmaceutical research · 2007Article
- An isolated in-situ rat head perfusion model for pharmacokinetic studies.Pharmaceutical research · 2000Article
- A physiological pharmacokinetic model for solute disposition in tissues below a topical below a topical application site.Pharmaceutical research · 1999Article
- Distribution and binding kinetics of ciprofloxacin and ofloxacin in the hindlimb of the rat.Pharmaceutical research · 1999Article
- A physiologically based pharmacokinetic model incorporating dispersion principles to describe solute distribution in the perfused rat hindlimb preparation.Journal of pharmacokinetics and biopharmaceutics · 1997Article
- The effects of perfusion conditions on melphalan distribution in the isolated perfused rat hindlimb bearing a human melanoma xenograft.British journal of cancer · 1997Article
- Tissue distribution kinetics as determinant of transit time dispersion of drugs in organs: application of a stochastic model to the rat hindlimb.Journal of pharmacokinetics and biopharmaceutics · 1996Article
- Discrepancies in pharmacokinetic parameter estimation between bolus and infusion studies in the perfused rat hindlimb.Journal of pharmacokinetics and biopharmaceutics · 1995Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Distribution of Evans Blue (EB), sucrose, and water into the isolated perfused rat hindlimb was studied under various conditions using the multiple indicator dilution (MID) technique. Statistical moment analysis of the outflow profiles for the EB, sucrose, and water were used to define the vascular, extravascular, and total water spaces, respectively. The varied perfusion conditions included albumin content (2, 4.7, and 7%), temperature (25, 37, and 42 C), perfusate flow rate (2, 4, 8, and 12 ml/min) and the presence/absence of red blood cells. The range of studies undertaken were chosen to represent the variety of conditions used in the preparation of both isolated animal and human limbs, the latter being particularly important in cytotoxic therapy for recurrent malignant melanoma. The distribution volumes of EB, sucrose, and water were dependent on the flow rate and the albumin content of perfusate. The normalized variances (CV2) of the markers were of the following order: sucrose (2.18) > water (1.58) > EB (0.68), indicating that some disequilibrium occurs during the capillary exchange of water and sucrose. It is suggested that a Krebs-Henseleit buffer containing 2% BSA is a suitable perfusate for most studies of the isolated rat hindlimb perfusion. The effect of albumin concentration manifests itself only at higher flows.
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