ArticleThe Journal of cell biology1994
Kalinin is more efficient than laminin in promoting adhesion of primary keratinocytes and some other epithelial cells and has a different requirement for integrin receptors.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
47 citing papers in PubMed, 255 citations in OpenAlex.
- Dermal stiffness governs the topography of the epidermis and the underlying basement membrane in young and old human skin.Aging cell · 2024Article
- Ultrasonographic Assessment of the Cutaneous Changes Induced by Topical Use of Novel Peptides Comprising Laminin 5.Archives of plastic surgery · 2022Article
- Burn Injury: Mechanisms of Keratinocyte Cell Death.Medical sciences (Basel, Switzerland) · 2021Review
- Perlecan expression influences the keratin 15-positive cell population fate in the epidermis of aging skin.Aging · 2016Article
- Molecular architecture and function of the hemidesmosome.Cell and tissue research · 2015Review
- Molecular architecture and function of the hemidesmosome.Cell and tissue research · 2015Review
- Cytoplasmic domain interactions of syndecan-1 and syndecan-4 with α6β4 integrin mediate human epidermal growth factor receptor (HER1 and HER2)-dependent motility and survival.The Journal of biological chemistry · 2014Article
- Expression of the gamma 2 chain of laminin-332 in eutopic and ectopic endometrium of patients with endometriosis.Reproductive biology and endocrinology : RB&E · 2013Article
- Cell surface proteoglycans syndecan-1 and -4 bind overlapping but distinct sites in laminin α3 LG45 protein domain.The Journal of biological chemistry · 2012Article
- Polymerized laminin-332 matrix supports rapid and tight adhesion of keratinocytes, suppressing cell migration.PloS one · 2012Article
- Adhesion and migration, the diverse functions of the laminin alpha3 subunit.Dermatologic clinics · 2010Review
- Tyrosine dephosphorylation of the syndecan-1 PDZ binding domain regulates syntenin-1 recruitment.The Journal of biological chemistry · 2009Article
- N-Glycosylation of laminin-332 regulates its biological functions. A novel function of the bisecting GlcNAc.The Journal of biological chemistry · 2008Article
- The short arm of laminin gamma2 chain of laminin-5 (laminin-332) binds syndecan-1 and regulates cellular adhesion and migration by suppressing phosphorylation of integrin beta4 chain.Molecular biology of the cell · 2007Article
- Plasma-membrane-associated sialidase (NEU3) differentially regulates integrin-mediated cell proliferation through laminin- and fibronectin-derived signalling.The Biochemical journal · 2006Article
- Laminin-5 (laminin-332): Unique biological activity and role in tumor growth and invasion.Cancer science · 2006Review
- Effects of constitutively active GTPases on fibroblast behavior.Cellular and molecular life sciences : CMLS · 2006Article
- The Rac activator Tiam1 is required for (alpha)3(beta)1-mediated laminin-5 deposition, cell spreading, and cell migration.The Journal of cell biology · 2005Article
- Activities of the matrix metalloproteinase stromelysin-2 (MMP-10) in matrix degradation and keratinocyte organization in wounded skin.Molecular biology of the cell · 2004Article
- Keratinocytes from patients lacking collagen XVII display a migratory phenotype.The American journal of pathology · 2004Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kalinin was purified from squamous cell carcinoma (SCC25) spent culture media using an immunoaffinity column prepared from the mAb BM165. The affinity-purified material was separated by SDS-PAGE into three bands of 165-155, 140, and 105 kD identical to those obtained from normal human keratinocyte cultures and previously identified as kalinin. Kalinin promoted adhesion of a large number of normal cells and established cell lines with an activity similar to other adhesion molecules such as the laminin-nidogen complex, fibronectin, or collagen IV. However, kalinin was a much better substrate than laminin-nidogen complex for adhesion of cells of epithelial origin including primary human keratinocytes. Adhesion to kalinin was followed by cell shape changes ranging from rounded to fully spread cells depending on the cell types. The adhesion-promoting activity of kalinin was conformation dependent and was abolished by heat denaturation. mAb BM165 prevented cell adhesion to kalinin but not to other extracellular matrix substrates. However, either complete or partial inhibition was observed with different cells suggesting the existence of at least two cell-binding sites on the kalinin molecule. Experiments inhibiting cell adhesion with function-blocking anti-integrin subunit antibodies indicated that both alpha 3 beta 1 and alpha 6 beta 1 integrins are involved in the cellular interactions with kalinin, while for cell adhesion to classical mouse Engelbreth-Holm-Swarm laminin only alpha 6 beta 1 integrins, and not alpha 3 beta 1, appeared to be functional. Altogether, these results suggest that kalinin may fulfill additional functions than laminin, particularly for epithelial cells.
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