ArticlePflugers Archiv : European journal of physiology1993
Modification of cardiac Ca2+ release channel gating by DIDS.
Article in Pflugers Archiv : European journal of physiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Transient receptor potential vanilloid channel 2 contributes to multi-modal endoplasmic reticulum and perinuclear space dilations that can also be observed in prion-infected mice.Acta neuropathologica · 2025Article
- The N-Terminal Region of the Ryanodine Receptor Affects Channel Activation.Frontiers in physiology · 2017Article
- Aldolase potentiates DIDS activation of the ryanodine receptor in rabbit skeletal sarcoplasmic reticulum.The Biochemical journal · 2006Article
- Luminal Ca2+-regulated Mg2+ inhibition of skeletal RyRs reconstituted as isolated channels or coupled clusters.The Journal of general physiology · 2004Article
- The Cl(-) channel blocker niflumic acid releases Ca(2+) from an intracellular store in rat pulmonary artery smooth muscle cells.British journal of pharmacology · 2003Article
- Regulation of the calcium release channel from skeletal muscle by suramin and the disulfonated stilbene derivatives DIDS, DBDS, and DNDS.Biophysical journal · 2003Article
- DIDS modifies the conductance, gating, and inactivation mechanisms of the cardiac ryanodine receptor.Biophysical journal · 2002Article
- Modification of ryanodine receptor/Ca2+ release channel with dinitrofluorobenzene.The Biochemical journal · 1999Article
- Identification of 30 kDa calsequestrin-binding protein, which regulates calcium release from sarcoplasmic reticulum of rabbit skeletal muscle.The Biochemical journal · 1998Article
- Voltage change-induced gating transitions of the rabbit skeletal muscle Ca2+ release channel.The Journal of physiology · 1998Article
- Pharmacological modulation of intracellular Ca(2+) channels at the single-channel level.Molecular neurobiologyReview
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Authors and funding
2 authors.
Funding
Abstract
The effects of 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) on individual cardiac sarcoplasmic reticulum Ca2+ release channels have been examined in planar lipid bilayers. A sudden step-like increase in open probability (Po) induced by DIDS was observed. At zero holding potential it proceeded with an apparent time constant of 84 s and Po was increased 15 times in the steady state. Independent of membrane voltage, DIDS induced a long-lived open state with tau o = 15 ms at 0 mV and tau o = 5 ms at -50 mV (cis (cytoplasmic) side negative). While at 0 mV the modified channel spent most of the time in this long-lived state, at -50 mV the occupancy of the long-lived state was very low and most openings occurred to the short and medium open state, leading to deactivation of the channel to only 2-3 times of control. Voltage-induced changes in the activity of modified channels were reversible and proceeded with time constants of 14 s for deactivation and 12 s for activation, that is, faster than onset of activation by DIDS. As no changes in single channel conductance of the channel were observed in the presence of DIDS and its abrupt activating action could not be removed, a covalent modification of an amino group residing in the gating structure of the channel by DIDS can be considered.
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Registered trials
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