ArticleThe Journal of cell biology1994
A novel laminin E8 cell adhesion site required for lung alveolar formation in vitro.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 82 citations in OpenAlex.
- Identification of skeletal muscle stem cell adhesion motifs using spot-synthesis-based peptide arrays.iScience · 2026Article
- Harnessing biomolecules for bioinspired dental biomaterials.Journal of materials chemistry. B · 2020Review
- In vitro model alveoli from photodegradable microsphere templates.Biomaterials science · 2015Article
- The laminin β1-competing peptide YIGSR induces a hypercontractile, hypoproliferative airway smooth muscle phenotype in an animal model of allergic asthma.Respiratory research · 2010Article
- Article
- An IKLLI-containing peptide derived from the laminin alpha1 chain mediating heparin-binding, cell adhesion, neurite outgrowth and proliferation, represents a binding site for integrin alpha3beta1 and heparan sulphate proteoglycan.The Biochemical journal · 1999Article
- Characterization and expression of the laminin gamma3 chain: a novel, non-basement membrane-associated, laminin chain.The Journal of cell biology · 1999Article
- The alpha5beta1 integrin mediates elimination of amyloid-beta peptide and protects against apoptosis.The Journal of cell biology · 1998Article
- Communication between the cell membrane and the nucleus: role of protein compartmentalization.Journal of cellular biochemistry. Supplement · 1998Review
- Laminin alpha1 chain synthesis in the mouse developing lung: requirement for epithelial-mesenchymal contact and possible role in bronchial smooth muscle development.The Journal of cell biology · 1997Article
- The laminin-binding activity of the alpha 7 integrin receptor is defined by developmentally regulated splicing in the extracellular domain.Molecular biology of the cell · 1997Article
- Reversion of the malignant phenotype of human breast cells in three-dimensional culture and in vivo by integrin blocking antibodies.The Journal of cell biology · 1997Article
- The extracellular matrix in epithelial biology: shared molecules and common themes in distant phyla.Developmental biology · 1996Review
- Inhibition of laminin alpha 1-chain expression leads to alteration of basement membrane assembly and cell differentiation.The Journal of cell biology · 1996Article
- CD44/chondroitin sulfate proteoglycan and alpha 2 beta 1 integrin mediate human melanoma cell migration on type IV collagen and invasion of basement membranes.Molecular biology of the cell · 1996Article
- Laminin mediates tissue-specific gene expression in mammary epithelia.The Journal of cell biology · 1995Article
- Antibodies against domain E3 of laminin-1 and integrin alpha 6 subunit perturb branching epithelial morphogenesis of submandibular gland, but by different modes.The Journal of cell biology · 1995Article
- Lacritin proteoforms prevent tear film collapse and maintain epithelial homeostasis.The Journal of biological chemistryArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Basement membrane-adherent type II alveolar cells isolated from lung assemble into lumen-containing cellular spheres which retain the correct polarity and thereby approximate the earliest fetal stage of alveolar morphogenesis. The molecular basis of this process, determined in initial experiments to be attributable mainly to the large heterotrimeric glycoprotein laminin, was probed with laminin proteolytic fragments, antibodies, and synthetic peptides. The carboxy-terminal fragment E8, but not equimolar amounts of fragment P1, blocked alveolar formation. To pursue this observation, we used several anti-E8 antibodies and identified one, prepared against A chain residues 2179-2198 ("SN-peptide") from the first loop of the G domain, as inhibitory. These results were confirmed by use of SN-peptide alone and further defined by trypsin digestion of SN-peptide to the sequence SINNNR. This conserved site promoted divalent cation dependent adhesion of both type II alveolar and HT1080 cells, was inhibitable with equimolar amounts of fragment E8 but not P1, and derives from a form of laminin present in fetal alveolar basement membranes. These studies point to an important novel cell adhesion site in the laminin E8 region with a key role in lung alveolar morphogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.