ArticleJournal of muscle research and cell motility1993
Flash photolysis studies of relaxation and cross-bridge detachment: higher sensitivity of tonic than phasic smooth muscle to MgADP.
Article in Journal of muscle research and cell motility, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 96 citations in OpenAlex.
- Effects of cytochalasin D on relaxation process of skinned taenia cecum and carotid artery from guinea pig.The journal of physiological sciences : JPS · 2024Article
- Molecular-level evidence of force maintenance by smooth muscle myosin during LC20 dephosphorylation.The Journal of general physiology · 2022Article
- Signaling and metabolic properties of fast and slow smooth muscle types from mice.Pflugers Archiv : European journal of physiology · 2018Article
- Smooth muscle-protein translocation and tissue function.Anatomical record (Hoboken, N.J. : 2007) · 2014Review
- Unphosphorylated calponin enhances the binding force of unphosphorylated myosin to actin.Biochimica et biophysica acta · 2013Article
- Differential expression of multidrug resistance protein 5 and phosphodiesterase 5 and regulation of cGMP levels in phasic and tonic smooth muscle.American journal of physiology. Gastrointestinal and liver physiology · 2013Article
- Ablation of smooth muscle caldesmon affects the relaxation kinetics of arterial muscle.Pflugers Archiv : European journal of physiology · 2013Article
- SMB myosin heavy chain knockout enhances tonic contraction and reduces the rate of force generation in ileum and stomach antrum.American journal of physiology. Cell physiology · 2013Article
- Smooth muscle heavy meromyosin phosphorylated on one of its two heads supports force and motion.The Journal of biological chemistry · 2009Article
- Affinity for MgADP and force of unbinding from actin of myosin purified from tonic and phasic smooth muscle.American journal of physiology. Cell physiology · 2008Article
- Molecular basis of the catch state in molluscan smooth muscles: a catchy challenge.Journal of muscle research and cell motility · 2008Review
- Regulation of the smooth muscle contractile phenotype by nonmuscle myosin.Journal of muscle research and cell motility · 2007Review
- Nonmuscle myosin, force maintenance, and the tonic contractile phenotype in smooth muscle.Pflugers Archiv : European journal of physiology · 2006Article
- Myosin regulatory light chain phosphorylation and strain modulate adenosine diphosphate release from smooth muscle Myosin.Biophysical journal · 2004Article
- Nonmuscle Myosin motor of smooth muscle.The Journal of general physiology · 2003Article
- The smooth muscle myosin seven amino acid heavy chain insert's kinetic role in the crossbridge cycle for mouse bladder.The Journal of physiology · 2003Article
- Smooth muscle and NMR review: an overview of smooth muscle metabolism.Molecular and cellular biochemistry · 2003Review
- Smooth, slow and smart muscle motors.Journal of muscle research and cell motility · 2003Review
- The kinetic properties of smooth muscle: how a little extra weight makes myosin faster.Journal of muscle research and cell motility · 2003Review
- Decreased shortening velocity and altered myosin isoforms in guinea-pig hypertrophic intestinal smooth muscle.The Journal of physiology · 2002Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The effects of MgADP and inorganic phosphate (Pi) on cross-bridge detachment were determined in tonic (rabbit femoral artery) and phasic (rabbit bladder and guinea pig portal vein) smooth muscles permeabilized with staphylococcal alpha-toxin. Relaxation from rigor was induced by photolysis of ATP (1.2-1.5 mM) from caged ATP. The initial one second of relaxation from rigor was resolved into two exponential components: a rapid component with normalized amplitudes, Af, of 8, 15 and 26% and rate constants, kf (in s-1) of 26, 36 and 30 in rabbit femoral artery, guinea pig portal vein, and rabbit bladder; the respective rate constants of the second, slower component, ks, were 0.07, 0.2 and 0.1. Removal of residual endogenous ADP with apyrase treatment increased the amplitude Af and accelerated ks; addition of MgADP reduced Af. The combination of these effects (increases in Af and ks) decreased the t1/2 of relaxation from control values by factors of 2.6 (femoral artery), 6.7 (portal vein) and 10 (bladder). Pi (30 mM) further increased the amplitudes Af. The affinity of MgADP for myosin cross-bridges, estimated as the reduction of the relative amplitude of the rapid component, Af, was significantly higher in tonic than in phasic smooth muscle: the KD of MgADP was 1.1 +/- 0.3 microM in rabbit femoral artery and 4.9 +/- 1.0 microM in rabbit bladder. The higher affinity of tonic smooth muscle myosin for MgADP correlated with its relatively high LC17b isoform content (58 +/- 4.2%) in contrast to the lower affinity of the phasic, bladder detrusor smooth muscle that contained only the LC17a isoform.(ABSTRACT TRUNCATED AT 250 WORDS)
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