ArticleJournal of clinical microbiology1994
Sandwich enzyme-linked immunosorbent assay for detection of excretory secretory antigens in humans with fascioliasis.
Article in Journal of clinical microbiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 92 citations in OpenAlex.
- Endemic human fasciolosis in the Bolivian Altiplano.Epidemiology and infection · 2007Trial
- The Detection of Circulating Antigen Glutathione S-Transferase in Sheep Infected withAnimals : an open access journal from MDPI · 2024Article
- Field evaluation of the enhanced MM3-COPRO ELISA test for the diagnosis of Fasciola hepatica infection in sheep.PloS one · 2022Article
- Bilious mask: parasite masquerading as malignant gall bladder polyp.BMJ case reports · 2021Article
- Recent Developments in Recombinant Proteins for Diagnosis of Human Fascioliasis.Acta parasitologica · 2021Review
- A sensitive serodiagnostic tool for the detection of active infection of zoonotic visceral and nasopharyngeal linguatulosis.Veterinary world · 2019Article
- Comparison of recombinant cathepsins L1, L2, and L5 as ELISA targets for serodiagnosis of bovine and ovine fascioliasis.Parasitology research · 2018Article
- Standardization of a latex agglutination test for coproantigen detection ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2018Article
- Rapid Enhanced MM3-COPRO ELISA for Detection of Fasciola Coproantigens.PLoS neglected tropical diseases · 2016Article
- Article
- Detection of Coproantigens by Sandwich ELISA in Rabbits Experimentally Infected with Fasciola gigantica.Iranian journal of parasitology · 2014Article
- Prevalence and risk factors for intestinal parasitic infections in a rural community in "consolación del sur" municipality, cuba.The West Indian medical journal · 2014Article
- Fascioliasis and intestinal parasitoses affecting schoolchildren in Atlixco, Puebla State, Mexico: epidemiology and treatment with nitazoxanide.PLoS neglected tropical diseases · 2013Article
- Immunodetection of Fasciola gigantica circulating antigen in sera of infected individuals for laboratory diagnosis of human fascioliasis.Clinical and vaccine immunology : CVI · 2013Article
- Immunodiagnosis of fascioliasis using sandwich enzyme-linked immunosorbent assay for detection of Fasciola gigantica paramyosin antigen.Tropical parasitology · 2013Article
- Evaluation of a 14.5 kDa-Fasciola gigantica fatty acid binding protein as a diagnostic antigen for human fascioliasis.Parasitology research · 2012Article
- Field evaluation of a coproantigen detection test for fascioliasis diagnosis and surveillance in human hyperendemic areas of Andean countries.PLoS neglected tropical diseases · 2012Article
- Diagnostic efficacy of monoclonal antibody based sandwich enzyme linked immunosorbent assay (ELISA) for detection of Fasciola gigantica excretory/secretory antigens in both serum and stool.Parasites & vectors · 2011Article
- A coproantigen diagnostic test for Strongyloides infection.PLoS neglected tropical diseases · 2011Article
- Fasciola hepatica infection in a 65-year-old woman.Journal of radiology case reports · 2010Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A sandwich enzyme-linked immunosorbent assay has been developed for the detection of Fasciola hepatica excretory secretory (ES) antigens in stool specimens of infected humans. The assay uses antibodies against F. hepatica ES antigens. A monoclonal antibody (ES78, mouse immunoglobulin G2a) was used to capture ES antigens, and a rabbit polyclonal antibody, peroxidase conjugate, was used to identify ES antigens. Thirteen of 14 patients with parasitological evidence of fascioliasis had a detectable concentration of ES antigens (more than 15 ng/ml). None of the stool specimens from controls and from patients with parasites other than F. hepatica showed a positive reaction, suggesting the absence of cross-reactions in this assay. When the 14 patients were retested 2 months after treatment, all of the specimens from the 11 parasitologically cured patients were negative by the antigen detection assay while the specimens from the 3 patients with persisting F. hepatica eggs in their stools remained positive.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.