ArticleJournal of clinical microbiology1994
Diagnostic value of anti-Candida enolase antibodies.
Article in Journal of clinical microbiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 63 citations in OpenAlex.
- Diagnosis of invasive candidiasis in neutropenic children with cancer by determination of D-arabinitol/L-arabinitol ratios in urine.Journal of clinical microbiology · 1997Trial
- A novel quantitative double antigen sandwich ELISA for detecting total antibodies against Candida albicans enolase 1.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Molecular cloning, expression, and purification, along withFrontiers in fungal biology · 2024Article
- Generation and Characterization of Single Chain Variable Fragment against Alpha-Enolase ofInternational journal of molecular sciences · 2020Article
- Reaction of antibodies toWorld journal of gastroenterology · 2019Article
- Advances in Candida detection platforms for clinical and point-of-care applications.Critical reviews in biotechnology · 2017Review
- The multifaceted roles of metabolic enzymes in the Paracoccidioides species complex.Frontiers in microbiology · 2014Review
- Diagnostic value of immunoglobulin G antibodies against Candida enolase and fructose-bisphosphate aldolase for candidemia.BMC infectious diseases · 2013Article
- Use of recombinant antigens for the diagnosis of invasive candidiasis.Clinical & developmental immunology · 2008Review
- Evaluation of a novel enzyme-linked immunosorbent assay to detect immunoglobulin G antibody to enolase for serodiagnosis of invasive candidiasis.Clinical and vaccine immunology : CVI · 2007Article
- Cloning, recombinant expression and activity studies of a major allergen "enolase" from the fungus Curvularia lunata.Journal of clinical immunology · 2006Article
- Simple and rapid detection of Candida albicans DNA in serum by PCR for diagnosis of invasive candidiasis.Journal of clinical microbiology · 2000Article
- New enzyme immunoassays for sensitive detection of circulating Candida albicans mannan and antimannan antibodies: useful combined test for diagnosis of systemic candidiasis.Journal of clinical microbiology · 1999Article
- Improved immunodiagnosis of human candidiasis by an enzyme-linked immunosorbent assay using a Candida albicans 52-kilodalton metallopeptidase.Clinical and diagnostic laboratory immunology · 1998Article
- The cell wall-associated glyceraldehyde-3-phosphate dehydrogenase of Candida albicans is also a fibronectin and laminin binding protein.Infection and immunity · 1998Article
- Cell wall and secreted proteins of Candida albicans: identification, function, and expression.Microbiology and molecular biology reviews : MMBR · 1998Review
- Serologic response to cell wall mannoproteins and proteins of Candida albicans.Clinical microbiology reviews · 1998Review
- Detection of antibodies to Candida albicans germ tubes for diagnosis and therapeutic monitoring of invasive candidiasis in patients with hematologic malignancies.Journal of clinical microbiology · 1997Article
- Differential humoral response against alpha- and beta-linked mannose residues associated with tissue invasion by Candida albicans.Clinical and diagnostic laboratory immunology · 1997Article
- Detection of candidal antigens in autoimmune polyglandular syndrome type I.Clinical and diagnostic laboratory immunology · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An immunodominant antigen with enolase enzyme activity was purified and used for the development of an assay to detect antibodies directed against this antigen in sera from patients with either invasive candidiasis or Candida colonization. The Au enzyme-linked immunosorbent assay established with the Candida enolase antigen was able to discriminate significantly between invasive candidiasis and colonization in both immunocompetent and immunodeficient groups of patients. The test had a sensitivity of 50% and a specificity of 86% in the immunocompetent patient group. In the immunodeficient patient group, a sensitivity of 53% and a specificity of 78% were established. Antibody levels determined by a counterimmunoelectrophoresis assay with the same set of sera resulted in a better sensitivity for sera from the immunocompetent patient group but a lower specificity, i.e., 80 and 29%, respectively. The counterimmunoelectrophoresis assay of sera from the immunodeficient patient group was not able to discriminate significantly between invasive candidiasis and colonization. With the use of more serum from each patient, the sensitivity of the antibody detection assays increased, while the specificity was maintained. The increase, however, was not statistically significant. Combining the results of the antibody assays with antigen titers obtained by the Cand-Tec assay did not improve the predictive value with respect to invasive candidiasis, as determined by multivariance regression analysis. Furthermore, it was demonstrated by performance of Western blots (immunoblots) that sera from patients as well as a rabbit antiserum cross-reacted with the Candida enolase and baker's yeast enolase enzyme. However, by tandem crossed immunoelectrophoresis it was demonstrated that the antibodies were directed toward different epitopes of the antigen.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.