ArticleNucleic acids research1994
AU-A, an RNA-binding activity distinct from hnRNP A1, is selective for AUUUA repeats and shuttles between the nucleus and the cytoplasm.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 77 citations in OpenAlex.
- Integrated functional and bioinformatics approach for the identification and experimental verification of RNA signals: application to HIV-1 INS.Nucleic acids research · 2003Article
- Overexpression of the 3'-untranslated region of myelin proteolipid protein mRNA leads to reduced expression of endogenous proteolipid mRNA.Neurochemical research · 2002Article
- RasGAP-associated endoribonuclease G3Bp: selective RNA degradation and phosphorylation-dependent localization.Molecular and cellular biology · 2001Article
- The role of 3'-untranslated region (3'-UTR) mediated mRNA stability in cardiovascular pathophysiology.Molecular and cellular biochemistry · 2001Article
- Polymorphism in the 3'-untranslated region of TNFalpha mRNA impairs binding of the post-transcriptional regulatory protein HuR to TNFalpha mRNA.Nucleic acids research · 2001Article
- Synchronous and regulated expression of two AU-binding proteins, AUF1 and HuR, throughout murine development.Oncogene · 2000Article
- Evidence that tristetraprolin binds to AU-rich elements and promotes the deadenylation and destabilization of tumor necrosis factor alpha mRNA.Molecular and cellular biology · 1999Article
- ELAV proteins stabilize deadenylated intermediates in a novel in vitro mRNA deadenylation/degradation system.Genes & development · 1999Article
- HNS, a nuclear-cytoplasmic shuttling sequence in HuR.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Overexpression of HuR, a nuclear-cytoplasmic shuttling protein, increases the in vivo stability of ARE-containing mRNAs.The EMBO journal · 1998Article
- Characterization of the RNA binding proteins forming complexes with a novel putative regulatory region in the 3'-UTR of TNF-alpha mRNA.Nucleic acids research · 1998Article
- Identification of a human VPF/VEGF 3' untranslated region mediating hypoxia-induced mRNA stability.Molecular biology of the cell · 1998Article
- AU-rich elements target small nuclear RNAs as well as mRNAs for rapid degradation.Genes & development · 1997Article
- Two different RNA binding activities for the AU-rich element and the poly(A) sequence of the mouse neuronal protein mHuC.Nucleic acids research · 1996Article
- Two distinct regions in the 3' untranslated region of tumor necrosis factor alpha mRNA form complexes with macrophage proteins.Molecular and cellular biology · 1996Article
- Complementary change in cis determinants and trans factors in the evolution of an mRNP stability complex.The EMBO journal · 1996Article
- Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells.Journal of virology · 1996Article
- Rapid degradation of AU-rich element (ARE) mRNAs is activated by ribosome transit and blocked by secondary structure at any position 5' to the ARE.Molecular and cellular biology · 1995Article
- mRNA decay mediated by two distinct AU-rich elements from c-fos and granulocyte-macrophage colony-stimulating factor transcripts: different deadenylation kinetics and uncoupling from translation.Molecular and cellular biology · 1995Article
- mRNA stability in mammalian cells.Microbiological reviews · 1995Review
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5 authors at 1 institution in 1 country.
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Abstract
The 3'-untranslated regions of many labile transcripts contain AU-rich sequences that serve as cis determinants of mRNA stability and translational efficiency. Using a photocrosslinking technique, our laboratory has previously defined three cytoplasmic RNA-binding activities specific for the AUUUA multimers found in the 3'-untranslated regions of lymphokine mRNAs. One of these activities, AU-A, has an apparent molecular mass of 34 kDa, is constitutively expressed in both primary T cells and the Jurkat T cell leukemia line, and binds to a variety of U-rich RNA sequences. Previous studies had shown that AU-A is more prevalent in the nucleus than the cytoplasm, raising the possibility that AU-A is really a nuclear RNA-binding activity that is found in cytoplasmic extracts because of nuclear leakage during cell fractionation. We now show that AU-A shuttles between the cytoplasm and the nucleus. Our results indicate that AU-A is a candidate protein component of ribonucleoprotein complexes that participate in nucleocytoplasmic transport of mRNA and cytoplasmic mRNA metabolism. The properties of AU-A activity are similar to those of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1). However, using monoclonal antibodies to hnRNP A1 and protease digestion patterns, we show that AU-A activity and hnRNP A1 protein are distinct. These studies have also allowed us to define a fourth RNA-binding activity of apparent molecular mass 41 kDa with specificity for AUUUA multimers. This activity is restricted to the nucleus and contains the hnRNP C protein.
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