ArticleNucleic acids research1994
Upstream and downstream cis-acting elements for cleavage at the L4 polyadenylation site of adenovirus-2.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Genomic Context and Insert Orientation in the Regulation of Transgene Expression in Adenoviral Vectors.International journal of molecular sciences · 2026Review
- Enzymatically amplified linear dbDNAGene therapy · 2023Article
- Sequence determinants of polyadenylation-mediated regulation.Genome research · 2019Article
- Recruitment of a basal polyadenylation factor by the upstream sequence element of the human lamin B2 polyadenylation signal.Molecular and cellular biology · 2000Article
- Assembly of the cleavage and polyadenylation apparatus requires about 10 seconds in vivo and is faster for strong than for weak poly(A) sites.Molecular and cellular biology · 1999Article
- Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis.Microbiology and molecular biology reviews : MMBR · 1999Review
- Polypyrimidine tract-binding protein positively regulates inclusion of an alternative 3'-terminal exon.Molecular and cellular biology · 1999Article
- The upstream sequence element of the C2 complement poly(A) signal activates mRNA 3' end formation by two distinct mechanisms.Genes & development · 1998Article
- Promoter-proximal poly(A) sites are processed efficiently, but the RNA products are unstable in the nucleus.Molecular and cellular biology · 1997Article
- Plant mRNA 3'-end formation.Plant molecular biology · 1996Review
- Efficient generation of recombinant adenovirus vectors by homologous recombination in Escherichia coli.Journal of virology · 1996Article
- A common mechanism for the enhancement of mRNA 3' processing by U3 sequences in two distantly related lentiviruses.Journal of virology · 1996Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A study of the cis-acting elements involved in the 3' end formation of the RNAs from the major late L4 family of adenovirus-2 was undertaken. Series of 5' or 3' end deletion mutants and mutants harboring either internal deletions or substitutions were prepared and assayed for in vitro cleavage. This first allowed the demonstration of a sequence, located at -6 to -29, relative to AAUAAA, whose deletion or substitution reduces cleavage efficiency at the L4 polyadenylation site two to three fold. This upstream efficiency element 5' AUCUUUGUUGUC/AUCUCUGUGCUG 3' is constituted of a partially repeated 12 nucleotide long, UCG rich sequence. The activities of the 2 sequence elements in cleavage are additive. We also searched for regulatory sequences downstream of the L4 polyadenylation site. We found that the deletion or substitution of a 30 nucleotide long UCG rich sequence, between nucleotides +7 and +35 relative to the cleavage site and harboring a UCCUGU repeat reduces cleavage efficiency at least ten fold. A GUUUUU sequence, starting at +35 had no influence. Thus, the usage of the L4 polyadenylation site requires down-stream sequences different from the canonical GU or U boxes and is regulated by upstream sequence elements.
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