ArticleNucleic acids research1994
Fine structure analyses of the Drosophila and Saccharomyces heat shock factor--heat shock element interactions.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
51 citing papers in PubMed, 169 citations in OpenAlex.
- Moesin contributes to heat shock gene response through direct binding to the Med15 subunit of the Mediator complex in the nucleus.Open biology · 2024Article
- Screening and Structural Characterization of Heat Shock Response Elements (HSEs) inInternational journal of molecular sciences · 2024Article
- The Plant-Beneficial Rhizobacterium Bacillus velezensis FZB42 Controls the Soybean Pathogen Phytophthora sojae Due to Bacilysin Production.Applied and environmental microbiology · 2021Article
- Heat Shock Factor Is Involved in Regulating the Transcriptional Expression of Two Potential Hsps (Frontiers in physiology · 2020Article
- Varying Architecture of Heat Shock Elements Contributes to Distinct Magnitudes of Target Gene Expression and Diverged Biological Pathways in Heat Stress Response of Bread Wheat.Frontiers in genetics · 2020Article
- FOXO3 on the Road to Longevity: Lessons From SNPs and Chromatin Hubs.Computational and structural biotechnology journal · 2019Review
- The Roles of Four Novel P450 Genes in Pesticides Resistance inFrontiers in genetics · 2019Article
- Metabolic control of the proteotoxic stress response: implications in diabetes mellitus and neurodegenerative disorders.Cellular and molecular life sciences : CMLS · 2016Review
- The evolution of heat shock protein sequences, cis-regulatory elements, and expression profiles in the eusocial Hymenoptera.BMC evolutionary biology · 2016Article
- UV Radiation and Visible Light Induce hsp70 Gene Expression in the Antarctic Psychrophilic Ciliate Euplotes focardii.Microbial ecology · 2015Article
- Complex regulation of Hsf1-Skn7 activities by the catalytic subunits of PKA in Saccharomyces cerevisiae: experimental and computational evidences.BMC systems biology · 2015Article
- Isolation of arginine kinase from Apis cerana cerana and its possible involvement in response to adverse stress.Cell stress & chaperones · 2015Article
- The expression profile and promoter analysis of ultraspiracle gene in the silkworm Bombyx mori.Molecular biology reports · 2014Article
- The expression profile and promoter analysis of β-N-acetylglucosaminidases in the silkworm Bombyx mori.Molecular biology reports · 2014Article
- Oxidative stress-related transcription factors in the regulation of secondary metabolism.Toxins · 2013Review
- Promoter- and RNA polymerase II-dependent hsp-16 gene association with nuclear pores in Caenorhabditis elegans.The Journal of cell biology · 2013Article
- Analyzing the promoters of two CYP9A genes in the silkworm Bombyx mori by dual-luciferase reporter assay.Molecular biology reports · 2013Article
- Identification and characterization of a novel calcyclin binding protein (CacyBP) gene from Apis cerana cerana.Molecular biology reports · 2012Article
- SNIP1: a new activator of HSE signaling pathway.Molecular and cellular biochemistry · 2012Article
- Inducible hsp70 in the regulation of cancer cell survival: analysis of chaperone induction, expression and activity.Cancers · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Heat shock genes are activated by the binding of the heat shock transcription factor (HSF) to heat shock elements (HSEs), consisting of arrays of the 5-bp unit NGAAN arranged as inverted repeats. Here, we have investigated the interaction of the 5-bp unit with HSFs of Drosophila and Saccharomyces. Mutations within the conserved, central trinucleotide GAA reduce the relative binding affinity of both HSFs. In addition, the base at position 1 (N1) also influences binding, with a strong preference for an A at this position. Methylation interference initially indicated that HSF contacts A1 in the minor groove, but interacts with the immediately adjacent base G2 in the major groove. Further characterization of this apparently abrupt minor to major groove transition by substitution of A1 with an inosine, shows that HSF contacts A1 in the major groove. We offer an explanation for this apparent contradiction and propose that HSF recognizes the HSE primarily through contacts within the major groove of the DNA helix. Finally, based on these observations and a re-evaluation of the base frequencies and criteria for consensus sequence assignment, we propose that the sequence AGAAN more accurately represents the consensus HSE motif.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.