ArticleThe Journal of cell biology1994
Okadaic acid disrupts clusters of synaptic vesicles in frog motor nerve terminals.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 113 citations in OpenAlex.
- Condensates of synaptic vesicles and synapsin-1 mediate actin sequestering and polymerization.The EMBO journal · 2025Article
- Condensates of synaptic vesicles and synapsin are molecular beacons for actin sequestering and polymerization.bioRxiv : the preprint server for biology · 2025Article
- Tau forms synaptic nano-biomolecular condensates controlling the dynamic clustering of recycling synaptic vesicles.Nature communications · 2023Article
- Frequency-Dependent Engagement of Synaptic Vesicle Pools in the Mice Motor Nerve Terminals.Cellular and molecular neurobiology · 2023Article
- Impaired Dopamine Release and Latent Learning in Alzheimer's Disease Model Zebrafish.ACS chemical neuroscience · 2022Article
- Multiple Roles of Actin in Exo- and Endocytosis.Frontiers in synaptic neuroscience · 2022Review
- Article
- Nanomachinery Organizing Release at Neuronal and Ribbon Synapses.International journal of molecular sciences · 2019Review
- Quantitative Determination of Cellular-and Neurite Motility Speed in Dense Cell Cultures.Frontiers in neuroinformatics · 2019Article
- Presynaptic morphology and vesicular composition determine vesicle dynamics in mouse central synapses.eLife · 2017Article
- Article
- Functionally heterogeneous synaptic vesicle pools support diverse synaptic signalling.The Journal of physiology · 2016Review
- Cholesterol regulates multiple forms of vesicle endocytosis at a mammalian central synapse.Journal of neurochemistry · 2015Article
- Is replenishment of the readily releasable pool associated with vesicular movement?Cognitive neurodynamics · 2014Article
- Spontaneous vesicle recycling in the synaptic bouton.Frontiers in cellular neuroscience · 2014Review
- Recovery of vesicular storage and release parameters after high frequency stimulation in rat hippocampus.Cognitive neurodynamics · 2013Article
- Identification of differentially expressed genes in SHSY5Y cells exposed to okadaic acid by suppression subtractive hybridization.BMC genomics · 2012Article
- Pre-aggregated Aβ1-42 peptide increases tau aggregation and hyperphosphorylation after short-term application.Molecular and cellular biochemistry · 2011Article
- High- and low-mobility stages in the synaptic vesicle cycle.Biophysical journal · 2010Article
- Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography.The Journal of cell biology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The fluorophore FM1-43 appears to stain membranes of recycled synaptic vesicles. We used FM1-43 to study mechanisms of synaptic vesicle clustering and mobilization in living frog motor nerve terminals. FM1-43 staining of these terminals produces a linear series of fluorescent spots, each spot marking the cluster of several hundred synaptic vesicles at an active zone. Most agents we tested did not affect staining, but the phosphatase inhibitor okadaic acid (OA) disrupted the fluorescent spots, causing dye to spread throughout the terminal. Consistent with this, electron microscopy showed that vesicle clusters were disrupted by OA treatment. However, dye did not spread passively to a uniform spatial distribution. Instead, time lapse movies showed clear evidence of active dye movements, as if synaptic vesicles were being swept along by an active translocation mechanism. Large dye accumulations sometimes occurred at sites of Schwann cell nuclei. These effects of OA were not significantly affected by pretreatment with colchicine or cytochalasin D. Electrophysiological recordings showed that OA treatment reduced the amount of acetylcholine released in response to nerve stimulation. The results suggest that an increased level of protein phosphorylation induced by OA treatment mobilizes synaptic vesicles and unmasks a powerful vesicle translocation mechanism, which may function normally to distribute synaptic vesicles between active zones.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.