ArticleThe Journal of cell biology1994
Two distinct, calcium-mediated, signal transduction pathways can trigger deflagellation in Chlamydomonas reinhardtii.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 94 citations in OpenAlex.
- A CRISPR activation screen reveals a cilia disassembly pathway mutated in focal cortical dysplasia.Science advances · 2025Article
- Cilia and Extracellular Vesicles in Brain Development and Disease.Biological psychiatry · 2024Review
- The younger flagellum sets the beat foreLife · 2024Article
- Determinants of cytoplasmic microtubule depolymerization during ciliogenesis inLife science alliance · 2024Article
- Shedding of ciliary vesicles at a glance.Journal of cell science · 2022Article
- Biosynthesis of Linear Protein Nanoarrays Using the Flagellar Axoneme.ACS synthetic biology · 2022Article
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- Responses to transient receptor potential (TRP) channel agonists inBiology open · 2020Article
- MKS-NPHP module proteins control ciliary shedding at the transition zone.PLoS biology · 2020Article
- Synchrony and symmetry-breaking in active flagellar coordination.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Primary cilium loss in mammalian cells occurs predominantly by whole-cilium shedding.PLoS biology · 2019Article
- A Flow Cytometry-Based Approach for the Isolation and Characterization of Neural Stem Cell Primary Cilia.Frontiers in cellular neuroscience · 2018Article
- Antagonistic bacteria disrupt calcium homeostasis and immobilize algal cells.Nature communications · 2017Article
- Spatial and temporal specificity of CaThe New phytologist · 2016Article
- A Forward Genetic Screen and Whole Genome Sequencing Identify Deflagellation Defective Mutants in Chlamydomonas, Including Assignment of ADF1 as a TRP Channel.G3 (Bethesda, Md.) · 2016Article
- Synergism between Inositol Polyphosphates and TOR Kinase Signaling in Nutrient Sensing, Growth Control, and Lipid Metabolism in Chlamydomonas.The Plant cell · 2016Article
- Mechanosensitive physiology of Chlamydomonas reinhardtii under direct membrane distortion.Scientific reports · 2014Article
- The awesome power of dikaryons for studying flagella and basal bodies in Chlamydomonas reinhardtii.Cytoskeleton (Hoboken, N.J.) · 2014Review
- A chemical screen identifies class a g-protein coupled receptors as regulators of cilia.ACS chemical biology · 2012Article
- Deciliation is associated with dramatic remodeling of epithelial cell junctions and surface domains.Molecular biology of the cell · 2009Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The molecular machinery of deflagellation can be activated in detergent permeabilized Chlamydomonas reinhardtii by the addition of Ca2+ (Sanders, M. A., and J. L. Salisbury, 1989. J. Cell Biol. 108:1751-1760). This suggests that stimuli which induce deflagellation in living cells cause an increase in the intracellular concentration of Ca2+, but this has never been demonstrated. In this paper we report that the wasp venom peptide, mastoparan, and the permeant organic acid, benzoate, activate two different signalling pathways to trigger deflagellation. We have characterized each pathway with respect to: (a) the requirement for extracellular Ca2+; (b) sensitivity to Ca2+ channel blockers; and (c) 45Ca influx. We also report that a new mutant strain of C. reinhardtii, adf-1, is specifically defective in the acid-activated signalling pathway. Both signalling pathways appear normal in another mutant, fa-1, that is defective in the machinery of deflagellation (Lewin, R. and C. Burrascano. 1983. Experientia. 39:1397-1398; Sanders, M. A., and J. L. Salisbury. 1989. J. Cell Biol. 108:1751-1760). We conclude that mastoparan induces the release of an intracellular pool of Ca2+ whereas acid induces an influx of extracellular Ca2+ to activate the machinery of deflagellation.
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