ArticleThe Journal of cell biology1994
Binding of pEL98 protein, an S100-related calcium-binding protein, to nonmuscle tropomyosin.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 157 citations in OpenAlex.
- Protein atlas of fibroblast specific protein 1 (FSP1)/S100A4.Histology and histopathology · 2023Article
- Molecular and Genetic Biomarkers in Idiopathic Pulmonary Fibrosis: Where Are We Now?Biomedicines · 2023Review
- Inhibition of the invasion and metastasis of mammary carcinoma cells by NBD peptide targeting S100A4 via the suppression of the Sp1/MMP‑14 axis.International journal of oncology · 2021Article
- Small Ones to Fight a Big Problem-Intervention of Cancer Metastasis by Small Molecules.Cancers · 2020Review
- Amlexanox Blocks the Interaction between S100A4 and Epidermal Growth Factor and Inhibits Cell Proliferation.PloS one · 2016Article
- Functional profile of S100A4-deficient T cells.Immunity, inflammation and disease · 2015Article
- Efficient inhibition of tumor angiogenesis and growth by a synthetic peptide blocking S100A4-methionine aminopeptidase 2 interaction.Molecular therapy. Methods & clinical development · 2015Article
- Joining S100 proteins and migration: for better or for worse, in sickness and in health.Cellular and molecular life sciences : CMLS · 2014Review
- Silencing of S100A4, a metastasis-associated protein, in endothelial cells inhibits tumor angiogenesis and growth.Angiogenesis · 2014Article
- Structure of the S100A4/myosin-IIA complex.BMC structural biology · 2013Article
- Proteome analysis of distinct developmental stages of human natural killer (NK) cells.Molecular & cellular proteomics : MCP · 2013Article
- The unique cytoplasmic domain of human FcγRIIIA regulates receptor-mediated function.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Crystal structure of the S100A4-nonmuscle myosin IIA tail fragment complex reveals an asymmetric target binding mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Rescue of tropomyosin deficiency in Drosophila and human cancer cells by synaptopodin reveals a role of tropomyosin α in RhoA stabilization.The EMBO journal · 2012Article
- Cysteine 81 is critical for the interaction of S100A4 and myosin-IIA.Biochemistry · 2011Article
- Two functional S100A4 monomers are necessary for regulating nonmuscle myosin-IIA and HCT116 cell invasion.Biochemistry · 2011Article
- Diagnostic and prognostic value of metastasis inducer S100A4 transcripts in plasma of colon, rectal, and gastric cancer patients.The Journal of molecular diagnostics : JMD · 2011Article
- S100A4 regulates macrophage chemotaxis.Molecular biology of the cell · 2010Article
- Phenothiazines inhibit S100A4 function by inducing protein oligomerization.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Self-association of calcium-binding protein S100A4 and metastasis.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cDNA coding for mouse fibroblast tropomyosin isoform 2 (TM2) was placed into a bacterial expression vector to produce a fusion protein containing glutathione-S-transferase (GST) and TM2 (GST/TM2). Glutathione-Sepharose beads bearing GST/TM2 were incubated with [35S]methionine-labeled NIH 3T3 cell extracts and the materials bound to the fusion proteins were analyzed to identify proteins that interact with TM2. A protein of 10 kD was found to bind to GST/TM2, but not to GST. The binding of the 10-kD protein to GST/TM2 was dependent on the presence of Ca2+ and inhibited by molar excess of free TM2 in a competition assay. The 10-kD protein-binding site was mapped to the region spanning residues 39-107 on TM2 by using several COOH-terminal and NH2-terminal truncation mutants of TM2. The 10-kD protein was isolated from an extract of NIH 3T3 cells transformed by v-Ha-ras by affinity chromatography on a GST/TM2 truncation mutant followed by SDS-PAGE and electroelution. Partial amino acid sequence analysis of the purified 10-kD protein, two-dimensional polyacrylamide gel analysis and a binding experiment revealed that the 10-kD protein was identical to a calcium-binding protein derived from mRNA named pEL98 or 18A2 that is homologous to S100 protein. Immunoblot analysis of the distribution of the 10-kD protein in Triton-soluble and -insoluble fractions of NIH 3T3 cells revealed that some of the 10-kD protein was associated with the Triton-insoluble cytoskeletal residue in a Ca(2+)-dependent manner. Furthermore, immunofluorescent staining of NIH 3T3 cells showed that some of the 10-kD protein colocalized with nonmuscle TMs in microfilament bundles. These results suggest that some of the pEL98 protein interacts with microfilament-associated nonmuscle TMs in NIH 3T3 cells.
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