ArticleMolecular and cellular biology1994
Large exon size does not limit splicing in vivo.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Longer internal exons tend to have more tandem repeats and more frequently experience insertions and deletions.Life science alliance · 2025Article
- Non-invasive and high-throughput interrogation of exon-specific isoform expression.Nature cell biology · 2021Article
- Programmable mutually exclusive alternative splicing for generating RNA and protein diversity.Nature communications · 2019Article
- An RNA Switch of a Large Exon of Ninein Is Regulated by the Neural Stem Cell Specific-RNA Binding Protein, Qki5.International journal of molecular sciences · 2019Article
- P_RNA_scaffolder: a fast and accurate genome scaffolder using paired-end RNA-sequencing reads.BMC genomics · 2018Article
- PASSPORT-seq: A Novel High-Throughput Bioassay to Functionally Test Polymorphisms in Micro-RNA Target Sites.Frontiers in genetics · 2018Article
- The kinetics of pre-mRNA splicing in theeLife · 2017Article
- Splicing of designer exons informs a biophysical model for exon definition.RNA (New York, N.Y.) · 2015Article
- Splicing of internal large exons is defined by novel cis-acting sequence elements.Nucleic acids research · 2012Article
- Challenging the spliceosome machine.Genome biology · 2006Article
- Computational definition of sequence motifs governing constitutive exon splicing.Genes & development · 2004Article
- Splicing of constitutive upstream introns is essential for the recognition of intra-exonic suboptimal splice sites in the thrombopoietin gene.Nucleic acids research · 2001Article
- Human genomic sequences that inhibit splicing.Molecular and cellular biology · 2000Article
- Architectural limits on split genes.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Selection of novel exon recognition elements from a pool of random sequences.Molecular and cellular biology · 1995Article
- Sequences homologous to 5' splice sites are required for the inhibitory activity of papillomavirus late 3' untranslated regions.Molecular and cellular biology · 1994Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Exon sizes in vertebrate genes are, with a few exceptions, limited to less than 300 bases. It has been proposed that this limitation may derive from the exon definition model of splice site recognition. In this model, a downstream donor site enhances splicing at the upstream acceptor site of the same exon. This enhancement may require contact between factors bound to each end of the exon; an exon size limitation would promote such contact. To test the idea that proximity was required for exon definition, we inserted random DNA fragments from Escherichia coli into a central exon in a three-exon dihydrofolate reductase minigene and tested whether the expanded exons were efficiently spliced. DNA from a plasmid library of expanded minigenes was used to transfect a CHO cell deletion mutant lacking the dhfr locus. PCR analysis of DNA isolated from the pooled stable cotransfectant populations displayed a range of DNA insert sizes from 50 to 1,500 nucleotides. A parallel analysis of the RNA from this population by reverse transcription followed by PCR showed a similar size distribution. Central exons as large as 1,400 bases could be spliced into mRNA. We also tested individual plasmid clones containing exon inserts of defined sizes. The largest exon included in mRNA was 1,200 bases in length, well above the 300-base limit implied by the survey of naturally occurring exons. We conclude that a limitation in exon size is not part of the exon definition mechanism.
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