ArticleMolecular and cellular biology1994
The heparin-binding domain of amphiregulin necessitates the precursor pro-region for growth factor secretion.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 58 citations in OpenAlex.
- Anti-EGFR Targeted Multifunctional I-131 Radio-Nanotherapeutic for Treating Osteosarcoma: In Vitro 3D Tumor Spheroid Model.Nanomaterials (Basel, Switzerland) · 2022Article
- The role of pro-domains in human growth factors and cytokines.Biochemical Society transactions · 2021Review
- Tetraspanins Function as Regulators of Cellular Signaling.Frontiers in cell and developmental biology · 2017Review
- Fractones: extracellular matrix niche controlling stem cell fate and growth factor activity in the brain in health and disease.Cellular and molecular life sciences : CMLS · 2016Review
- Amphiregulin activates regulatory T lymphocytes and suppresses CD8+ T cell-mediated anti-tumor response in hepatocellular carcinoma cells.Oncotarget · 2015Article
- Fractone-heparan sulphates mediate FGF-2 stimulation of cell proliferation in the adult subventricular zone.Cell proliferation · 2013Article
- Myofibroblast keratinocyte growth factor reduces tight junctional integrity and increases claudin-2 levels in polarized Caco-2 cells.Growth factors (Chur, Switzerland) · 2012Article
- Transautocrine signaling by membrane neuregulins requires cell surface targeting, which is controlled by multiple domains.The Journal of biological chemistry · 2011Article
- Amphiregulin exosomes increase cancer cell invasion.Current biology : CB · 2011Article
- Article
- Insights from bacterial subtilases into the mechanisms of intramolecular chaperone-mediated activation of furin.Methods in molecular biology (Clifton, N.J.) · 2011Review
- Amphiregulin as a novel target for breast cancer therapy.Journal of mammary gland biology and neoplasia · 2008Review
- The kinetics of the hydrogen/deuterium exchange of epidermal growth factor receptor ligands.Biophysical journal · 2008Article
- Maternal heparin-binding-EGF deficiency limits pregnancy success in mice.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Epidermal growth factor receptor signaling is required for normal ovarian steroidogenesis and oocyte maturation.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The membrane-anchoring domain of epidermal growth factor receptor ligands dictates their ability to operate in juxtacrine mode.Molecular biology of the cell · 2005Article
- Myristoylated Naked2 escorts transforming growth factor alpha to the basolateral plasma membrane of polarized epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- N-arginine dibasic convertase is a specific receptor for heparin-binding EGF-like growth factor that mediates cell migration.The EMBO journal · 2001Article
- Article
- Removal of the membrane-anchoring domain of epidermal growth factor leads to intracrine signaling and disruption of mammary epithelial cell organization.The Journal of cell biology · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The five members of the human epidermal growth factor (EGF) family (EGF, transforming growth factor alpha [TGF-alpha], heparin-binding EGF-like growth factor [HB-EGF], betacellulin, and amphiregulin [AR]) are synthesized as transmembrane proteins whose extracellular domains are proteolytically processed to release the biologically active mature growth factors. These factors all activate the EGF receptor, but in contrast to EGF and TGF-alpha, the mature forms of HB-EGF and AR are also glycosylated, heparin-binding proteins. We have constructed a series of mutants to examine the influence of the distinct precursor domains in the biosynthesis of AR. The transmembrane and cytoplasmic domains of the precursor are not required for secretion of bioactive AR from either COS or mammary epithelium-derived cells, although proteolytic removal of the N-terminal pro-region is less efficient in the absence of the membrane anchor. Deletion of the N-terminal pro-region, however, results in rapid intracellular degradation of the molecule with no detectable secretion of active growth factor. AR secretion is preserved by replacing the native pro-region with the corresponding domain of the HB-EGF precursor but not with that of the TGF-alpha precursor. In the absence of any N-terminal pro-region, secretion of the molecule is restored by deleting the N-terminal heparin-binding domain of mature AR. Both EGF and TGF-alpha, in contrast, can be secreted without their pro-regions. However, if the protein is fused with the AR heparin-binding domain, TGF-alpha secretion is inhibited unless the AR pro-region is also present. We propose that the heparin-binding domain of mature AR necessitates the presence of a specific structural motif in an N-terminal pro-region to permit proper folding, and thus secretion, of a bioactive molecule.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.